Cat. No.: HY-121793

Roemerine

Name: Roemerine
Brand: MedChemExpress
SKU: HY-121793
Price: 120.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: (-)-Roemerine)

고객리뷰

Based on 1 Customer Validation

Roemerine is an alkaloid that has been identified from the leaves of Fibraurea recisa Pierre. Roemerine exhibits antibacterial, anticancer, and antidepressant activities, can reverse the multidrug resistance phenotype in cultured cells, and exerts antibacterial effects by regulating the cAMP signaling pathway. Additionally, Roemerine influences neuronal activity by increasing BDNF protein expression and modulating the serotonergic and glutamatergic systems. Roemerine holds promise for research in the fields of cancer, infections, and neurological diseases.

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Purity: 99.89%
CAS No.: 548-08-3
화학식: C₁₈H₁₇NO₂
분자량:279.33

보관:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Roemerine Chemical Structure

CAS No.: 548-08-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
용액&용해도
- In Vitro
- In Vivo
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

All Endogenous Metabolite Isoforms :

All 5-HT Receptor Isoforms :

All mGluR Isoforms :

All iGluR Isoforms :

All Antibiotic Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	ED₅₀	4.3 μg/mL Compound: 1	Cytotoxicity against human A431 cells after 72 hrs by SRB assay Cytotoxicity against human A431 cells after 72 hrs by SRB assay	[PMID: 7623038]
BC1 cell line	ED₅₀	8.3 μg/mL Compound: 1	Cytotoxicity against human BC1 cells after 72 hrs by SRB assay Cytotoxicity against human BC1 cells after 72 hrs by SRB assay	[PMID: 7623038]
Col2	ED₅₀	5.8 μg/mL Compound: 1	Cytotoxicity against human Col2 cells after 72 hrs by SRB assay Cytotoxicity against human Col2 cells after 72 hrs by SRB assay	[PMID: 7623038]
HT	ED₅₀	6.4 μg/mL Compound: 1	Cytotoxicity against human HT cells after 72 hrs by SRB assay Cytotoxicity against human HT cells after 72 hrs by SRB assay	[PMID: 7623038]
KB-V1	ED₅₀	>20 μg/mL Compound: 1	Cytotoxicity against human multidrug resistant KBV1 cells in absence of vinblastine after 72 hrs by SRB assay Cytotoxicity against human multidrug resistant KBV1 cells in absence of vinblastine after 72 hrs by SRB assay	[PMID: 7623038]
KB-V1	ED₅₀	0.6 μg/mL Compound: 1	Cytotoxicity against human multidrug resistant KBV1 cells in presence of vinblastine Cytotoxicity against human multidrug resistant KBV1 cells in presence of vinblastine	[PMID: 7623038]
LNCaP	ED₅₀	10.7 μg/mL Compound: 1	Cytotoxicity against human LNCAP cells after 72 hrs by SRB assay Cytotoxicity against human LNCAP cells after 72 hrs by SRB assay	[PMID: 7623038]
Lu1	ED₅₀	6.6 μg/mL Compound: 1	Cytotoxicity against human Lu1 cells after 72 hrs by SRB assay Cytotoxicity against human Lu1 cells after 72 hrs by SRB assay	[PMID: 7623038]
P388	ED₅₀	>5 μg/mL Compound: 1	Cytotoxicity against mouse P388 cells after 72 hrs by SRB assay Cytotoxicity against mouse P388 cells after 72 hrs by SRB assay	[PMID: 7623038]
RAW264.7	IC₅₀	1.39 μM Compound: 130	Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay	[PMID: 33454546]
SK-MEL-2	ED₅₀	>20 μg/mL Compound: 1	Cytotoxicity against human SK-MEL-2 cells after 72 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells after 72 hrs by SRB assay	[PMID: 7623038]
U-373MG ATCC	ED₅₀	5 μg/mL Compound: 1	Cytotoxicity against human U373 cells after 72 hrs by SRB assay Cytotoxicity against human U373 cells after 72 hrs by SRB assay	[PMID: 7623038]
ZR-75-1	ED₅₀	>20 μg/mL Compound: 1	Cytotoxicity against human ZR-75-1 cells after 72 hrs by SRB assay Cytotoxicity against human ZR-75-1 cells after 72 hrs by SRB assay	[PMID: 7623038]

In Vitro

Roemerine (0-1 mM, 48 h) significantly reduces cell viability in RM-1 cells, with IC₅₀ of 1177.4 µM ^[2].
Roemerine (8 µg/mL, 8 h) can influence biofilm formation in Candida albicans SC5314 through the regulation of the cAMP signaling pathway^[3].
Roemerine (10 μM， 48 h) affects neuronal activity by increasing BDNF protein expression and modulating the serotonergic and glutamatergic systems in SH-SY5Y cells, exhibiting antidepressant-like effects^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

RM-1 cells (prostate cancer, mouse)
Concentration:

0 µM, 1 µM, 2 µM, 5 µM, 10 µM, 20 µM, 50 µM, 100 µM, 200 µM, 500 µM, 1000 µM
Incubation Time:

48 h
Result:

Significantly reduced RM-1 cell viability, with IC₅₀ of 1177.4 µM.

RT-PCR^[3]

Cell Line:

Candida albicans SC5314
Concentration:

8 µg/mL
Incubation Time:

8 h
Result:

Significantly upregulated YWP1, SAP5, SAP6, HWP1, and ECE1 genes while downregulating EFG1 gene.

In Vivo

Roemerine (30 mg/kg, i.p., once every three days for 30 days) significantly inhibited tumor growth in the prostate cancer BALB/C nude mouse model (established by subcutaneous implantation of RM-1 cells)^[2].
Roemerine (20 mg/kg, i.p., once daily for 2 days) increases survival and reduced bacterial loads in multiple organs in the MRSA XJ75302-induced sepsis BALB/C mouse model^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Prostate cancer BALB/C nude mouse model (established by subcutaneous implantation of RM-1 cells)^[3]
Dosage:

30 mg/kg
Administration:

Intraperitoneal injection (i.p.), once every three days for 30 days
Result:

Significantly reduced tumor volume and weight in the experimental group (P < 0.05), with an inhibition rate of 41.2%.

Animal Model:

MRSA XJ75302-induced sepsis BALB/C mouse model^[5]
Dosage:

20 mg/kg
Administration:

Intraperitoneal injection (i.p.), once daily, for 2 days
Result:

Increased the 7-day survival rate of mice to 58.3% (p < 0.05) and reduced bacterial loads in the liver (4.71 to 4.38 log CFU/mL), kidney (4.37 to 3.79 log CFU/mL), heart (5.44 to 3.76 log CFU/mL), and blood (6.52 to 4.55 log CFU/mL).
Note: CFU/mL represents the number of bacteria per milliliter of culture medium or tissue sample that can form colonies (CFU, Colony-Forming Units).

Chemical Information

CAS No. 548-08-3
Appearance Solid
분자량 279.33
화학식 C₁₈H₁₇NO₂
Color White to off-white
SMILES

CN1CCC(C2=C3C4=CC=CC=C4C[C@@]12[H])=CC5=C3OCO5
Synonyms

(-)-Roemerine
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
- Alkaloids
- Aporphine Alkaloids
Initial Source
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

용액&용해도

In Vitro:

DMSO : 100 mg/mL (358.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5800 mL

17.9000 mL

35.8000 mL

5 mM

0.7160 mL

3.5800 mL

7.1600 mL

10 mM

0.3580 mL

1.7900 mL

3.5800 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. M You, et al. (-)-Roemerine, an Aporphine Alkaloid From Annona Senegalensis That Reverses the Multidrug-Resistance Phenotype With Cultured Cells. J Nat Prod. 1995 Apr;58(4):598-604. [Content Brief]

[2]. Xu J, et al. Inhibition of proliferation, migration and invasion of RM-1 cells by roemerine: Insights from in vitro and in vivo studies. Tissue Cell. 2024 Dec 20;93:102693. [Content Brief]

[3]. Ma C, et al. Potent Activities of Roemerine against Candida albicans and the Underlying Mechanisms. Molecules. 2015 Sep 29;20(10):17913-28. [Content Brief]

[4]. Bayazeid O, et al. Neuroactivity of the naturally occurring aporphine alkaloid, roemerine. Nat Prod Res. 2021 Dec;35(24):6147-6152. [Content Brief]

[5]. Yin S, et al. Roemerine Improves the Survival Rate of Septicemic BALB/c Mice by Increasing the Cell Membrane Permeability of Staphylococcus aureus. PLoS One. 2015 Nov 25;10(11):e0143863. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5800 mL	17.9000 mL	35.8000 mL	89.4999 mL
	5 mM	0.7160 mL	3.5800 mL	7.1600 mL	17.9000 mL
	10 mM	0.3580 mL	1.7900 mL	3.5800 mL	8.9500 mL
	15 mM	0.2387 mL	1.1933 mL	2.3867 mL	5.9667 mL
	20 mM	0.1790 mL	0.8950 mL	1.7900 mL	4.4750 mL
	25 mM	0.1432 mL	0.7160 mL	1.4320 mL	3.5800 mL
	30 mM	0.1193 mL	0.5967 mL	1.1933 mL	2.9833 mL
	40 mM	0.0895 mL	0.4475 mL	0.8950 mL	2.2375 mL
	50 mM	0.0716 mL	0.3580 mL	0.7160 mL	1.7900 mL
	60 mM	0.0597 mL	0.2983 mL	0.5967 mL	1.4917 mL
	80 mM	0.0447 mL	0.2237 mL	0.4475 mL	1.1187 mL
	100 mM	0.0358 mL	0.1790 mL	0.3580 mL	0.8950 mL

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Roemerine

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Elacridar

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Elacridar (Standard)

Benzoylaconine

Roquefortine C

Piperine

Verapamil (Standard)

Tariquidar

Coniferyl ferulate

Piperine (Standard)

Valspodar

All Endogenous Metabolite Isoforms

All 5-HT Receptor Isoforms

All mGluR Isoforms

All iGluR Isoforms

All Antibiotic Isoforms

Biological Activity

Chemical Information

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

Roemerine

Voacamine

Elacridar

Verapamil

Elacridar (Standard)

Benzoylaconine

Roquefortine C

Piperine

Verapamil (Standard)

Tariquidar

Coniferyl ferulate

Piperine (Standard)

Valspodar

All Endogenous Metabolite Isoforms

All 5-HT Receptor Isoforms

All mGluR Isoforms

All iGluR Isoforms

All Antibiotic Isoforms

Biological Activity

Chemical Information

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

Roemerine Related Classifications

HY-121793 Related Classifications

Bioz