Cat. No.: HY-N1198

Strictosamide

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Strictosamide is a compound that can be isolated from Nauclea officinalis. Strictosamide has various activities such as anti-inflammatory, analgesic, anti-Plasmodium, antifungal, and promoting wound healing.

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Pureté: 99.94%
CAS No.: 23141-25-5
Formule: C₂₆H₃₀N₂O₈
Masse moléculaire:498.53

Stockage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Strictosamide Chemical Structure

CAS No.: 23141-25-5

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Activité biologique
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

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Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>80 μM Compound: 21	Cytotoxicity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 31804070]
HepG2	IC₅₀	>80 μM Compound: 21	Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 31804070]
MCF7	IC₅₀	>80 μM Compound: 21	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 31804070]

In Vitro

Strictosamide (0-200 μM; 24 h) can inhibit the production of NO, TNF-α, and IL-1β, as well as the expression of iNOS in LPS (HY-D1056)-treated RAW 264.7 cells. The mechanism involves the inhibition of the NF-κB and MAPK pathways^[1].
Strictosamide (0.25-2 mg/mL) can inhibit the Mg²⁺-ATPase activity in isolated kidneys^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

LPS (HY-D1056)-treated RAW 264.7 cells
Concentration:

0, 25, 50, 100 and 200 µM
Incubation Time:

24 h
Result:

Markedly decreased the expressions of p-p65, p-IκBα and p-IKKα in NF-κB signaling pathways.
Significantly decreased the phosphorylation of p38, ERK and JNK in MAPK signaling pathways.

In Vivo

Strictosamide (50-200 mg/kg; intraperitoneal injection; single dose) can increase the Na⁺, K⁺-ATPase activity in the mouse brain and inhibit the Mg²⁺-ATPase activity in the mouse kidneys^[2].
Strictosamide (10-40 mg/kg; intravenous injection; twice a day; 3 days) has anti-inflammatory and analgesic effects in multiple mouse models^[3].
Strictosamide (0.5-1.5 mg/kg; topical application; twice a day; 8 days) has a wound-healing-promoting effect in a rat wound model. The mechanism involves the activation of the PI3K/AKT signaling pathway^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

ICR mice, weighing 18-22 g^[3]
Dosage:

10, 20 and 40 mg/kg
Administration:

Intravenous injection; twice a day; 3 days
Result:

Obviously decreased the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mice ear edema (24.7 and 28.1% inhibition, respectively) at 20 and 40 mg/kg.
Significantly inhibited acetic acid (HY-Y0319)-stimulated peritoneal vascular permeability in mice (23.3 and 33.4% inhibition, respectively) at 20 and 40 mg/kg.
Significantly decreased the leukocytes in the mice peritoneal cavity induced by CMC–Na at all the tested doses (46.0, 49.1, and 58.7% inhibition, respectively).
Markedly prolonged the pain latency at 20 and 40 mg/kg and decreased the writhing counts at 40 mg/kg (49.7% inhibition) in acetic acid (HY-Y0319)-induced writhing test in mice. However, it did not obviously improve the pain threshold of mice in hot-plate test.

Animal Model:

Male Sprague Dawley (SD) rats (200-220 g) with skin wound model^[4]
Dosage:

0.5 and 1.5 mg/kg
Administration:

Topical application; twice a day; 8 days
Result:

Promoted wound healing.
Reduced inflammatory infiltration.
Enhanced collagen deposition.
Upregulated the expression of CD31 and PCNA

Chemical Information

CAS No. 23141-25-5
Appearance Solid
Masse moléculaire 498.53
Formule C₂₆H₃₀N₂O₈
Color Off-white to yellow
SMILES

O=C1N2CCC3=C(NC4=C3C=CC=C4)[C@]2([H])C[C@]5([H])C1=CO[C@@H](O[C@H]6[C@@H]([C@H]([C@@H]([C@@H](CO)O6)O)O)O)[C@@H]5C=C
Structure Classification
- Alkaloids
- Indole Alkaloids
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (200.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0059 mL

10.0295 mL

20.0590 mL

5 mM

0.4012 mL

2.0059 mL

4.0118 mL

10 mM

0.2006 mL

1.0029 mL

2.0059 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (280 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Li D, et al. Anti-inflammatory effect of the six compounds isolated from Nauclea officinalis Pierrc ex Pitard, and molecular mechanism of strictosamide via suppressing the NF-κB and MAPK signaling pathway in LPS-induced RAW 264.7 macrophages. J Ethnopharmacol. 2017 Jan 20;196:66-74. [Content Brief]

[2]. Candeias MF, et al. Effects of strictosamide on mouse brain and kidney Na+, K+-ATPase and Mg2+-ATPase activities. J Ethnopharmacol. 2009 Jan 12;121(1):117-22. [Content Brief]

[3]. Na Li, et al. In vivo anti-inflammatory and analgesic activities of strictosamide from Nauclea officinalis. Pharm Biol. 2014 Nov;52(11):1445-50. [Content Brief]

[4]. Ming GX, et al. Strictosamide promotes wound healing through activation of the PI3K/AKT pathway. Heliyon. 2024 Apr 23;10(9):e30169. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0059 mL	10.0295 mL	20.0590 mL	50.1474 mL
	5 mM	0.4012 mL	2.0059 mL	4.0118 mL	10.0295 mL
	10 mM	0.2006 mL	1.0029 mL	2.0059 mL	5.0147 mL
	15 mM	0.1337 mL	0.6686 mL	1.3373 mL	3.3432 mL
	20 mM	0.1003 mL	0.5015 mL	1.0029 mL	2.5074 mL
	25 mM	0.0802 mL	0.4012 mL	0.8024 mL	2.0059 mL
	30 mM	0.0669 mL	0.3343 mL	0.6686 mL	1.6716 mL
	40 mM	0.0501 mL	0.2507 mL	0.5015 mL	1.2537 mL
	50 mM	0.0401 mL	0.2006 mL	0.4012 mL	1.0029 mL
	60 mM	0.0334 mL	0.1672 mL	0.3343 mL	0.8358 mL
	80 mM	0.0251 mL	0.1254 mL	0.2507 mL	0.6268 mL
	100 mM	0.0201 mL	0.1003 mL	0.2006 mL	0.5015 mL

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Strictosamide

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