Doxycycline-d3 hydrochloride
Doxycycline-d3 hydrochloride is the deuterated-labeled Doxycycline hydrochloride (HY-N0565A). Doxycycline hydrochloride is an orally active, broad-spectrum tetracycline antibiotic that also functions as an MMP inhibitor, exhibiting antibacterial, antiviral, antiparasitic, and antitumor activities. Doxycycline hydrochloride can bind to the classical binding site (CBS) within the mRNA decoding center of the 30S small subunit of the bacterial 70S ribosome.Doxycycline hydrochloride blocks bacterial protein synthesis and inhibits the degradation of the extracellular matrix through its interaction with zinc and calcium ions. Doxycycline hydrochloride also inhibits mitochondrial biogenesis, translation, and the expression of respiratory chain proteins. Doxycycline hydrochloride induces apoptosis, inhibits autophagy and EMT, downregulates stem cell markers, and activates the PI3K-AKT pathway, thereby effectively inhibiting the viability and proliferation of cancer cells such as breast cancer cells. Doxycycline hydrochloride can disrupt transthyretin (TTR) amyloid fibrils. Doxycycline hydrochloride also promotes the survival and self-renewal of embryonic stem cells and neural stem cells, and reduces the frequency of medium changes in culture. Doxycycline hydrochloride can be used in research related to cancers—such as breast, prostate, and bladder cancer—as well as neurological disorders.
For research use only. We do not sell to patients.
- Formula: C22H22D3ClN2O8
- Molecular Weight:483.91
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
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Tetracycline |
PI3K |
Akt |
MMP-8 |
MMP-9 |
30S subunit |
In Vitro
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- Research Protocol for Infectious Diseases
- RNA extraction experimental
- Collagen: Sirius Red Staining
- Mitochondrial membrane-potential fluorescent assay
- Annexin V plus membrane-impermeant dye apoptosis staining
- ECM-Embedded Organoid (Matrigel/Dome) Culture
- Breast Cancer Modeling
- Amyloid: Congo Red Amyloid Staining
- Matrigel Transwell/Boyden Chamber Invasion Assay
- Cell Viability Determination by MTT Colorimetric Assay
- Fibrosis/Collagen Morphometry
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Mitochondrial membrane-potential and mitochondrial mass staining
- Autophagy
- Apoptosis Solutions
- Matrigel/ECM Transwell Invasion Assay
- Lysosome and acidic-vesicle live-cell staining
- Macroautophagy Solutions
- hPSC maintenance and expansion
Application
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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Unlabeled CAS 10592-13-9
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Molecular Weight 483.91
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Formula C22H22D3ClN2O8
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SMILES
O=C(C(C1=O)=C(O)[C@H](N(C([2H])([2H])[2H])C)[C@@]2([H])[C@H](O)[C@@]3([H])[C@H](C)C4=C(C(C3=C(O)[C@]21O)=O)C(O)=CC=C4)N.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. El-Neweshy MS, et al. Experimental doxycycline overdose in rats causes cardiomyopathy. Int J Exp Pathol. 2013;94(2):109-114. [Content Brief]
[2]. Zhang L, et al. Doxycycline inhibits the cancer stem cell phenotype and epithelial-to-mesenchymal transition in breast cancer. Cell Cycle. 2017;16(8):737-745. [Content Brief]
[5]. Chang MY, et al. Doxycycline supplementation allows for the culture of human ESCs/iPSCs with media changes at 3-day intervals. Stem Cell Res. 2015;15(3):608-613. [Content Brief]
[6]. Manfredsson FP, et al. Tight Long-term dynamic doxycycline responsive nigrostriatal GDNF using a single rAAV vector. Mol Ther. 2009 Nov;17(11):1857-67. [Content Brief] [Content Brief]
[7]. Kistner A, et al. Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10933-8. [Content Brief]
[8]. Niv Y. Doxycycline in Eradication Therapy of Helicobacter pylori--a Systematic Review and Meta-Analysis. Digestion. 2016;93(2):167-73. [Content Brief]
Hot Topics
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Topics Stem Cells & Neurodegenerative Diseases
Stem cells possess self-renewal and multilineage differentiation capacities, making them a central platform for regenerative medicine and neurodegenerative disease research.
2026-06-18
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Doxycycline-d3 hydrochloride Related Classifications
HY-N0565AS Related Classifications
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Keywords
- Doxycycline-d3
- Isotope-Labeled Compounds
- Antibiotic
- MMP
- Parasite
- Bacterial
- Apoptosis
- Akt
- PI3K
- Transthyretin (TTR)
- Matrix metalloproteinases
- PKB
- Protein kinase B
- Phosphoinositide 3-kinase
- thyroxine-binding prealbumin
- antibacterial activity
- anti-proliferative activity
- antibiotic
- LNT-229
- G55
- U343
- SVG
- MCF 12A
- 293T
- Inhibitor
- inhibitor
- inhibit