Hispidin
Based on 1 publication(s) in Google Scholar
Hispidin, a PKC inhibitor and a phenolic compound can be found in Phellinus linteus, has been shown to possess strong anti-oxidant, anti-cancer, anti-diabetic, and anti-dementia properties.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.85%
- No. CAS: 555-55-5
- Fòrmula: C13H10O5
- Peso molecular:246.22
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Hispidin
MoreVer todos los productos específicos de isoformas Endogenous Metabolite
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Actividad biológica
Descripciòn
IC50 & Target
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Microbial Metabolite |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| MDCK | CC50 |
>100 μM
Compound: 1
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Cytotoxicity against MDCK cells by SRB assay
Cytotoxicity against MDCK cells by SRB assay
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[PMID: 26077494] |
| MDCK | IC50 |
57.9 μM
Compound: 1
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Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect by SRB assay
Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect by SRB assay
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[PMID: 26077494] |
In Vitro
Hispidin (20 μM, 24 hours) alleviates ferroptosis and improves pancreatic β-cell function (as evidenced by the inhibition of ROS, Fe²⁺, and MDA accumulation, along with an increase in GSH levels), primarily exerting its protective effects through the regulation of the miR-15b-5p/GLS2 axis[2]. Hispidin (20 μg/ml, 24 hours) mitigates LPS (HY-D1056)-induced inflammatory responses in macrophages by inhibiting the phosphorylation of the MAPK and JAK1/STAT3 signaling pathways[3]. Hispidin (20 µM, 25 hours) protects dopaminergic neurons from JNK activation-regulated mitochondrial-dependent apoptosis[4]. Hispidin (3-30 μM, 7 hours) protects against hydrogen peroxide-induced apoptosis in H9c2 cardiomyocytes via the Akt/GSK-3β and ERK1/2 signaling pathways[5]. Hispidin (2.5-20 μM, 24 hours) exerts a protective effect against hydrogen peroxide-induced oxidative stress in ARPE-19 cells through the Nrf2 signaling pathway[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Mouse pancreatic β-cell line Min6
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Concentration:20, 40, 60 μM
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Incubation Time:24 h
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Result:Significantly increased cell viability and had a protective effect under high glucose conditions.
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Cell Line:Mouse pancreatic β-cell line Min6
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Concentration:20, 40, 60 μM
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Incubation Time:24 h
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Result:Significantly reduced GLS2 expression.
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Cell Line:LPS-induced RAW264.7 macrophage cells
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Concentration:20 μg/mL
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Incubation Time:20 min (followed by LPS (1 μg/ml) treatment for 24 hours)
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Result:Significantly inhibited the phosphorylation of p-ERK, p-JNK, p-p38 (MAPK pathway-related) and p-JAK1, p-STAT3 (JAK1/STAT3 pathway-related) induced by LPS.
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Cell Line:H9c2 cardiomyoblast cells (rat cardiomyocytes)
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Concentration:3, 10, 30 μM
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Incubation Time:7 h (1-hour pretreatment, followed by 6-hour H2O2 (600 μM) exposure.)
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Result:Significantly reduced H2O2-induced Bax and caspase-3 levels, increased Bcl-2 expression, and decreased apoptotic cell percentage.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Skin wound model: excision wounds, dead space wounds, linear incision wounds (Wistar rats)[7]
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Dosage:2.5% (w/w) and 5% (w/w) Hispidin ointment
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Administration:Topical application, 0.2 g daily, for 14 days
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Result:Enhanced wound contraction, accelerated epithelialization, promoted tissue regeneration, fibroblast activity, and angiogenesis. Reduced pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), and increased anti-inflammatory cytokines (IL-10) and antioxidant enzymes (SOD, GPx, CAT).
Chemical Information
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No. CAS 555-55-5
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Appearance Solid
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Peso molecular 246.22
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Fòrmula C13H10O5
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Color Light yellow to yellow
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SMILES
O=C1C=C(O)C=C(/C=C/C2=CC=C(O)C(O)=C2)O1
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Biomedicines
The Efficacy of Hispidin and Magnesium Nanoparticles against Zearalenone-Induced Fungal Toxicity Causing Polycystic Ovarian Syndrome in Rats. [Abstract]2024 Apr 24;12(5):943. PMID: 38790905
Solvente y solubilidad
In Vitro:
DMSO : 19.23 mg/mL (78.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.92 mg/mL (7.80 mM); Clear solution
This protocol yields a clear solution of ≥ 1.92 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (19.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.92 mg/mL (7.80 mM); Clear solution
This protocol yields a clear solution of ≥ 1.92 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (19.2 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Kim DE, et al. The protective effect of hispidin against hydrogen peroxide-induced apoptosis in H9c2 cardiomyoblast cells through Akt/GSK-3β and ERK1/2 signaling pathway. Exp Cell Res. 2014 Oct 1;327(2):264-75. [Content Brief]
[2]. Wu F, et al. Hispidin Inhibits Ferroptosis Induced by High Glucose via the miR-15b-5p/GLS2 Axis in Pancreatic Beta Cells. Evid Based Complement Alternat Med. 2023 Feb 21;2023:9428241. [Content Brief]
[4]. Lai MC, et al. Hispidin in the Medicinal Fungus Protects Dopaminergic Neurons from JNK Activation-Regulated Mitochondrial-Dependent Apoptosis in an MPP+-Induced In Vitro Model of Parkinson's Disease. Nutrients. 2023 Jan 20;15(3):549. [Content Brief]
[5]. Kim DE, et al. The protective effect of hispidin against hydrogen peroxide-induced apoptosis in H9c2 cardiomyoblast cells through Akt/GSK-3β and ERK1/2 signaling pathway. Exp Cell Res. 2014 Oct 1;327(2):264-75. [Content Brief]
[6]. Huang SY, et al. The Protective Effect of Hispidin against Hydrogen Peroxide-Induced Oxidative Stress in ARPE-19 Cells via Nrf2 Signaling Pathway. Biomolecules. 2019 Aug 19;9(8):380. [Content Brief]
[7]. Liu YS, et al. Exploring the Wound Healing Potential of Hispidin. Nutrients. 2024 Sep 19;16(18):3161. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.0614 mL | 20.3070 mL | 40.6141 mL | 101.5352 mL |
| 5 mM | 0.8123 mL | 4.0614 mL | 8.1228 mL | 20.3070 mL | |
| 10 mM | 0.4061 mL | 2.0307 mL | 4.0614 mL | 10.1535 mL | |
| 15 mM | 0.2708 mL | 1.3538 mL | 2.7076 mL | 6.7690 mL | |
| 20 mM | 0.2031 mL | 1.0154 mL | 2.0307 mL | 5.0768 mL | |
| 25 mM | 0.1625 mL | 0.8123 mL | 1.6246 mL | 4.0614 mL | |
| 30 mM | 0.1354 mL | 0.6769 mL | 1.3538 mL | 3.3845 mL | |
| 40 mM | 0.1015 mL | 0.5077 mL | 1.0154 mL | 2.5384 mL | |
| 50 mM | 0.0812 mL | 0.4061 mL | 0.8123 mL | 2.0307 mL | |
| 60 mM | 0.0677 mL | 0.3385 mL | 0.6769 mL | 1.6923 mL |
Hispidin Related Classifications
HY-100618 Related Classifications
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