Cat. No.: HY-N0631

Cornuside

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

Cornuside is an iridoid glycoside with anti-allergic, anti-inflammatory, and neuroprotective activities. Cornuside exerts anti-allergic activity by downregulating the p38 MAPK, JNK, and NF-κB signaling pathways, and inhibits IgE-mediated histamine release from mast cells. Cornuside improves cognitive impairment in mice by inhibiting BACE1 activity (IC₅₀ = 55.84 μg/mL) and enhancing ChAT activity. Cornuside inhibits LPS (HY-D1056)-induced inflammatory mediators, including iNOS, COX-2, PGE2, TNF-α, IL-6, and IL-1β, by suppressing NF-κB activation.

For research use only. We do not sell to patients.

Purity: 99.99%
CAS No.: 131189-57-6
Formula: C₂₄H₃₀O₁₄
Molecular Weight:542.49

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Cornuside Chemical Structure

CAS No.: 131189-57-6

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[2]^[3]^[1]

p38 MAP kinase

NF-κB

JNK1

JNK2

AChE

BChE

iNOS

IL-6

IL-1β

COX-2

All p38 MAPK Isoforms :

All NF-κB Isoforms :

All JNK Isoforms :

All Cholinesterase (ChE) Isoforms :

AChE
BChE

All Acyltransferase Isoforms :

All NO Synthase Isoforms :

All Interleukin Related Isoforms :

All TNF Receptor Isoforms :

All Prostaglandin Receptor Isoforms :

All ERK Isoforms :

All COX Isoforms :

In Vitro

Cornuside exhibits free radical scavenging activity in a cell-free DPPH assay with an IC₅₀ of 20 μM^[1].
Cornuside protects SH-SY5Y cells from H₂O₂-induced damage by enhancing endogenous antioxidant defense systems^[1].
Cornuside (3-30 μM; 30 min) dose-dependently inhibits anti-DNP IgE-mediated histamine release from rat peritoneal mast cells, with significant effects at 10 and 30 μM^[2].
Cornuside (3-30 μM; 4 h) dose-dependently inhibits the production and secretion of TNF-α and IL-6 by PMA/A23187-stimulated human mast cells (HMC-1), with significant effects at 10 and 30 μM^[2].
Cornuside (3-30 μM; 1 h pre-incubation, 18 h LPS stimulation) dose-dependently inhibits LPS-induced nitric oxide production in RAW 264.7 cells, with maximum inhibition of 67.6% at 30 μM after 18 hours of LPS stimulation^[3].
Cornuside (3-30 μM; 1 h pre-incubation, 1 h LPS stimulation) dose-dependently inhibits LPS-induced nuclear translocation of the NF-κB p65 subunit in RAW 264.7 cells after 1 hour of LPS stimulation, with maximum inhibition at 30 μM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

human mast cells (HMC-1)
Concentration:

3, 10 and 30 μM
Incubation Time:

4 h (during PMA/A23187 stimulation)
Result:

Inhibited protein expression and secretion of TNF-α and IL-6 in a dose-dependent manner, with significant inhibition observed at 10 μM and 30 μM.\n
Inhibited the activation of p38 and JNK in a dose-dependent manner.
Reduced NF-κB p65 levels in the nucleus while increasing NF-κB p65 levels in the cytosol of PMA/A23187-stimulated HMC-1 cells.

ELISA Assay^[3]

Cell Line:

LPS-stimulated RAW 264.7 macrophage cell line
Concentration:

3, 10, 30 μM
Incubation Time:

1 h pre-incubation, followed by 18 h incubation with LPS
Result:

Significantly attenuated LPS-induced PGE2 production in a dose-dependent manner.

Western Blot Analysis^[3]

Cell Line:

LPS-stimulated RAW 264.7 macrophage cell line
Concentration:

3, 10, 30 μM
Incubation Time:

1 h pre-incubation, followed by 18 h incubation with LPS
Result:

Concentration-dependently suppressed LPS-induced protein expressions of iNOS and COX-2.

RT-PCR^[3]

Cell Line:

LPS-stimulated RAW 264.7 macrophage cell line
Concentration:

3, 10, 30 μM
Incubation Time:

1 h pre-incubation, followed by 6 h incubation with LPS
Result:

Concentration-dependently suppressed LPS-induced mRNA expressions of iNOS and COX-2, with levels correlated to protein expression changes.

Western Blot Analysis^[3]

Cell Line:

LPS-stimulated RAW 264.7 macrophage cell line
Concentration:

3, 10, 30 μM
Incubation Time:

1 h pre-incubation, followed by 1 h incubation with LPS
Result:

Dose-dependently inhibited LPS-induced translocation of the p65 subunit of NF-κB from the cytosol to the nucleus.\n
Increased cytoplasmic p65 levels to 80% of vehicle control and decreased nuclear p65 levels to 30% of LPS-only control at 30 μM.
Significantly blocked LPS-stimulated phosphorylation and degradation of IkB-α in a dose-dependent manner.\n
Reduced p-IkB-α levels to 20% of LPS-only control and restored IkB-α levels to 90-100% of vehicle control at 10 and 30 μM.\nSuppressed LPS-stimulated phosphorylations of ERK1/2, p38, and JNK1/2 in a concentration-dependent manner.\n
Reduced p-ERK1/2 levels to 40%, p-p38 levels to 10-15%, and p-JNK1/2 levels to 10-15% of LPS-only control at 30 μM.\n
Left non-phosphorylated ERK1/2, p38, and JNK1/2 expression unaffected.

In Vivo

Cornuside (3-30 mg/kg; p.o.; daily administration for 15 consecutive days) ameliorates scopolamine (HY-N0296)-induced cognitive impairment in mice^[1].
Cornuside (50-200 mg/kg; p.o.; single administration) dose-dependently inhibits passive cutaneous anaphylaxis in mice and rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

ICR mice (male, 20 g, scopolamine-induced amnesia)^[1]
Dosage:

3 mg/kg; 10 mg/kg; 30 mg/kg
Administration:

p.o.; daily; day 4 to day 19
Result:

Significantly improved the learning and memory impairments of mice in the Scopolamine model in water maze, dark avoidance and jumping platform experiments.

Animal Model:

Male Sprague-Dawley and Institute of Cancer Research mice^[2]
Dosage:

50 mg/kg; 100 mg/kg; 200 mg/kg
Administration:

p.o.; single dose 1 hour before antigen challenge
Result:

Produced dose-dependent inhibition of passive cutaneous anaphylaxis.

Chemical Information

CAS No. 131189-57-6
Appearance Solid
Molecular Weight 542.49
Formula C₂₄H₃₀O₁₄
Color White to off-white
SMILES

O=C(C1=CC(O)=C(O)C(O)=C1)OCCC(C(C(OC)=O)=COC2OC(C(C(O)C3O)O)OC3CO)C2C=C
Structure Classification
- Terpenoids
- Iridoids
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (7)

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Journal Impact Factor

Phytomedicine

Cornuside improves murine autoimmune hepatitis through inhibition of inflammatory responses. [Abstract]

2023 Nov:120:155077. PMID: 37716032
Free Radic Biol Med

Cornuside alleviates microglia-mediated neuroinflammation to ameliorate chronic neuropathic pain-induced cognitive impairment via the Nrf2/Sirt3 signaling pathway. [Abstract]

2026 Jun:249:292-307. PMID: 41831801
Drug Des Devel Ther

The Natural Compound Cornuside Protects Against Acute Liver Failure Induced by Lipopolysaccharide and D-Galactosamine. [Abstract]

2026 Jun 3:20:601517. PMID: 42261449
Cells

Identification of Natural Compounds Triggering MRGPRX2-Mediated Calcium Flux and Degranulation in RBL-2H3 Cells. [Abstract]

2026 Feb 3;15(3):287. PMID: 41677650
Int Immunopharmacol

Cornuside alleviates psoriasis-like skin lesions in mice by relieving inflammatory effects. [Abstract]

2024 Jun 15:134:112183. PMID: 38705031
Naunyn Schmiedebergs Arch Pharmacol

Inhibitory effects of cornuside on human liver cytochrome P450 enzymes. [Abstract]

2025 Feb 13. PMID: 39939490
Tohoku J Exp Med

Cornuside Ameliorates Diabetic Nephropathy Possibly by Regulating Angiogenesis and MAPK Signaling. [Abstract]

2025 May 28;266(1):87-95. PMID: 39443135

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (184.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8434 mL

9.2168 mL

18.4335 mL

5 mM

0.3687 mL

1.8434 mL

3.6867 mL

10 mM

0.1843 mL

0.9217 mL

1.8434 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wang ZX, et al. Cornuside ameliorates cognitive impairments in scopolamine induced AD mice: Involvement of neurotransmitter and oxidative stress. J Ethnopharmacol. 2022;293:115252. [Content Brief]

[2]. Li L, et al. Cornuside inhibits mast cell-mediated allergic response by down-regulating MAPK and NF-κB signaling pathways. Biochem Biophys Res Commun. 2016;473(2):408-414. [Content Brief]

[3]. Choi YH, et al. Cornuside suppresses lipopolysaccharide-induced inflammatory mediators by inhibiting nuclear factor-kappa B activation in RAW 264.7 macrophages. Biol Pharm Bull. 2011;34(7):959-966. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8434 mL	9.2168 mL	18.4335 mL	46.0838 mL
	5 mM	0.3687 mL	1.8434 mL	3.6867 mL	9.2168 mL
	10 mM	0.1843 mL	0.9217 mL	1.8434 mL	4.6084 mL
	15 mM	0.1229 mL	0.6145 mL	1.2289 mL	3.0723 mL
	20 mM	0.0922 mL	0.4608 mL	0.9217 mL	2.3042 mL
	25 mM	0.0737 mL	0.3687 mL	0.7373 mL	1.8434 mL
	30 mM	0.0614 mL	0.3072 mL	0.6145 mL	1.5361 mL
	40 mM	0.0461 mL	0.2304 mL	0.4608 mL	1.1521 mL
	50 mM	0.0369 mL	0.1843 mL	0.3687 mL	0.9217 mL
	60 mM	0.0307 mL	0.1536 mL	0.3072 mL	0.7681 mL
	80 mM	0.0230 mL	0.1152 mL	0.2304 mL	0.5760 mL
	100 mM	0.0184 mL	0.0922 mL	0.1843 mL	0.4608 mL

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Cornuside

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Publications Citing Use of MedChemExpress (MCE) Cornuside

All p38 MAPK Isoforms

All NF-κB Isoforms