Pomalidomide
Based on 39 publication(s) in Google Scholar
Pomalidomide (CC-4047) is an immunomodulatory agent. Pomalidomide downregulates TNF-alpha, IL-1, IL-11, and IL-12, upregulates IL-2, IL-5, and IL-10, and costimulates T cells. Pomalidomide inhibits the proliferation of T regulatory cells by reducing FOXP3/OX-40 expression and IL-2-mediated expansion. Pomalidomide inhibits CDK2/4/6 and p21WAF-1, causing cell cycle arrest. Pomalidomide can be used in research on myelofibrosis-associated anemia, multiple myeloma, and colorectal cancer.
For research use only. We do not sell to patients.
- Purity : 99.88%
- CAS No.: 19171-19-8
- Formula: C13H11N3O4
- Molecular Weight:273.24
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
Publications Citing Use of MedChemExpress (MCE) Pomalidomide
More- Cancer Cell. 2022 Nov 14;40(11):1294-1305.e4. [Abstract]
- Cell. 2021 Oct 14;184(21):5375-5390.e16. [Abstract]
- Nat Cancer. 2022 May;3(5):595-613. [Abstract]
- Cancer Res. 2025 Oct 28. [Abstract]
- Nat Commun. 2024 Mar 16;15(1):2377. [Abstract]
- Nat Commun. 2022 Sep 10;13(1):5324. [Abstract]
- Nat Commun. 2017 May 22:8:15398. [Abstract]
- Acta Pharm Sin B. 2026 Feb 10.
- Adv Sci (Weinh). 2026 Jul 17:e76662.
- Cell Rep Med. 2025 Apr 15;6(4):102053. [Abstract]
- Biomaterials. 2022 Oct:289:121800. [Abstract]
- J Transl Med. 2024 Dec 3;22(1):1095. [Abstract]
- Mol Ther Oncol. 2025 Feb 20;33(1):200952. [Abstract]
- Cell Rep. 2026 Jul 28;45(8):117762.
- J Med Chem. 2024 Oct 24;67(20):18247-18264. [Abstract]
- Drug Des Devel Ther. 2026 Jan 22;20:1-21.
- Eur J Pharmacol. 2025 Dec 5:1008:178303. [Abstract]
- Int Immunopharmacol. 2025 Jun 17:158:114831. [Abstract]
- Cancers (Basel). 2024 Mar 28;16(7):1319. [Abstract]
- Structure. 2026 Mar 20:S0969-2126(26)00056-0. [Abstract]
- iScience. 2023 Jun 7;26(7):107059. [Abstract]
- Clin Exp Immunol. 2021 Jul;205(1):53-62. [Abstract]
- Gen Comp Endocrinol. 2016 Mar 1:228:1-8. [Abstract]
- Stem Cells Dev. 2025 Mar 25. [Abstract]
- Biomed Pharmacother. 2026 Sep:202:119803.
- SSRN. 2026 Jul 13.
- bioRxiv. 2026 May 21:2026.05.19.726384. [Abstract]
- bioRxiv. 2026 Feb 8:2026.02.06.704516. [Abstract]
- Charles University. 2026.
- Chemrxiv. 2025 Nov 17.
- Harvard University. 2025.
- bioRxiv. 2025 Jun 19.
- bioRxiv. 2025 January 08.
- Patent. US20240245677A1.
- University of Colorado Denver. 2024.
- University of Colorado Denver. 2024.
- bioRxiv. 2024 Jan 28.
- Elife. 2018 Aug 1:7:e38430. [Abstract]
- Masaryk University. 2014.
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WB
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All Orexin Receptor (OX Receptor) Isoforms
More
Biological Activity
Description
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IL-1 |
TNF-alpha |
IL-12 |
IL-2 |
IL-5 |
IL-10 |
IL-11 |
FOXP3 |
CDK2 |
OX-40 |
CDK4 |
CDK6 |
Cereblon |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| OPM-2 | IC50 |
0.038 μM
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Antiproliferative activity against human OPM-2 multiple myeloma cells assessed by [3H]-thymidine incorporation after 3-day culture.
Antiproliferative activity against human OPM-2 multiple myeloma cells assessed by [3H]-thymidine incorporation after 3-day culture.
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23203815 |
| NCI-H929 | IC50 |
0.035 μM
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Antiproliferative activity against human H929 multiple myeloma cells assessed by [3H]-thymidine incorporation after 3-day culture.
Antiproliferative activity against human H929 multiple myeloma cells assessed by [3H]-thymidine incorporation after 3-day culture.
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23203815 |
| U-266 | IC50 |
0.028 μM
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Antiproliferative activity against human U266 multiple myeloma cells assessed by [3H]-thymidine incorporation after 3-day culture.
Antiproliferative activity against human U266 multiple myeloma cells assessed by [3H]-thymidine incorporation after 3-day culture.
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23203815 |
In Vitro
Pomalidomide eliminates the suppressive function of human T regulatory cells on autologous CD4+CD25− responder cells[3].
Pomalidomide (0.001-10 μM; 24 h) reduces FOXP3 expression in purified human T regulatory cells, with an inhibition rate of up to 50% at 10 μM[3].
Pomalidomide (0.001-1 μM; 24 h) inhibits OX40 expression on purified human T regulatory cells at concentrations as low as 0.01 μM without affecting GITR[3].
Pomalidomide (24 h)-induced G0-G1 phase arrest in Namalwa cells requires p21WAF-1[4].
Pomalidomide (0.001-100 µM; 6 days) expands CD34+ progenitor cells and increases p21WAF-1 without inhibiting CDK2 activity[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Namalwa Burkitt’s lymphoma cells
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Concentration:0.1, 1, and 10 µM (cell cycle); 0.001, 0.01, 0.1, 1, and 10 µM (cell number/apoptosis); 0.01, 0.1, 1, and 10 µM (cell number, days 3, 4, and 5)
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Incubation Time:72 h (cell cycle); 72 h (cell number/apoptosis); days 3, 4, and 5 (cell number)
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Result:Increased G0-G1 phase cells to 53.1% at 0.1 µM, 72.3% at 1 µM, and 79.5% at 10 µM, with corresponding decreases in S-phase cells to 35.2%, 18.3%, and 15.3%.
Decreased Namalwa cell number dose-dependently without significantly affecting apoptosis.
Produced a 4-fold decrease in cell number at 10 µM by day 5.
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Cell Line:Namalwa cells
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Concentration:0.01, 0.1, 1, and 10 µM (p21WAF-1 mRNA/protein); 0.01, 0.1, 1, and 10 µM (CDK immunoprecipitation and pRb phosphorylation)
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Incubation Time:8, 24, and 72 h (p21WAF-1 mRNA/protein); 24 h (CDK immunoprecipitation and pRb phosphorylation)
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Result:Increased p21WAF-1 mRNA and protein in a dose- and time-dependent manner.
Enhanced p21WAF-1 binding to CDK2, CDK4, and CDK6 and decreased pRb phosphorylation at Ser795, Ser608, Ser780, Ser612, and Thr821 without changing total pRb protein.
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Cell Line:Namalwa cells
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Concentration:0.001, 0.01, 0.1, 1, and 10 µM; VPA 0.1, 0.3, and 1 mM
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Incubation Time:3 days
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Result:At 0.1 mM, VPA was additive, whereas at 1 mM, VPA was synergistic in inducing Namalwa cell apoptosis.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (female, approximately 12-15 weeks old)[3]
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Dosage:50 mg/kg
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Administration:i.p.; daily; 6 days
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Result:In draining lymph nodes, CD4+CD25+CTLA-4+FOXP3+ T regulatory cells were reduced by 40% compared with the PBS control.
T regulatory cells averaged 11% of the CD4 population in non-tumor-challenged mice and increased to 18% after CT26 challenge; draining lymph node T regulatory cells were reduced to levels seen in non-tumor-challenged animals.
No significant reduction in T regulatory cells was seen in the spleen.
CD25−CD4+ cells or total CD4+ lymphocyte numbers in lymph nodes or spleen were not affected.
Chemical Information
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CAS No. 19171-19-8
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Appearance Solid
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Molecular Weight 273.24
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Formula C13H11N3O4
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Color Light yellow to yellow
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SMILES
O=C1N(C(C2=C1C=CC=C2N)=O)C(C(N3)=O)CCC3=O
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Synonyms
CC-4047; Actimid
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (39)
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Journal Impact Factor
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Most Recent
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Cancer Cell
2022 Nov 14;40(11):1294-1305.e4. PMID: 36084652 -
Cell
2021 Oct 14;184(21):5375-5390.e16. PMID: 34562363 -
Nat Cancer
IKAROS and MENIN coordinate therapeutically actionable leukemogenic gene expression in MLL-r acute myeloid leukemia. [Abstract]2022 May;3(5):595-613. PMID: 35534777
Pomalidomide purchased from MedChemExpress. Usage Cited in: Nat Cancer. 2022 May;3(5):595-613. [Abstract]
Western blot analysis for IKAROS, CK1α and MENIN protein following treatment of MV4;11 human MLL-r AML cell line for 5 hours with increasing doses of Thalidomide, lenalidomide, Pomalidomide and iberdomide (10,100, 1000 nM), using ACTIN as a loading control.
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Cancer Res
CFT1946 is an Orally Available Brain-Penetrant BRAFV600-Mutant Degrader that Overcomes BRAF Inhibitor Resistance. [Abstract]2025 Oct 28. PMID: 41150906 -
Nat Commun
2024 Mar 16;15(1):2377. PMID: 38493213 -
Nat Commun
Functional genomics uncovers the transcription factor BNC2 as required for myofibroblastic activation in fibrosis. [Abstract]2022 Sep 10;13(1):5324. PMID: 36088459
Pomalidomide purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Sep 10;13(1):5324. [Abstract]
Western immunoassays used to monitor changes in BNC2 levels in LX2 cells treated with 1 μM pomalidomide (Pom.), thalidomide (Thal.), CC-885, Iberdomide (Iber.) or vehicle for 24 h.
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Nat Commun
pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4CRBN ubiquitin ligase. [Abstract]2017 May 22:8:15398. PMID: 28530236
Pomalidomide purchased from MedChemExpress. Usage Cited in: Nat Commun. 2017 May 22:8:15398. [Abstract]
NUDT1 immunoblots using SF188 cells pretreated with pomalidomide (10 µM) for 2 h, and then subjected to an additional 6 h treatment with DMSO or LC-1-40 (50 nM).
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Cell Rep Med
CAN-Scan: A multi-omic phenotype-driven precision oncology platform identifies prognostic biomarkers of therapy response for colorectal cancer. [Abstract]2025 Apr 15;6(4):102053. PMID: 40187357 -
Biomaterials
2022 Oct:289:121800. PMID: 36166893 -
J Transl Med
Doxorubicin synergizes bortezomib-induced multiple myeloma cell death by inhibiting aggresome formation and augmenting endoplasmic reticulum/Golgi stress and apoptosis. [Abstract]2024 Dec 3;22(1):1095. PMID: 39623468 -
Mol Ther Oncol
Dual CARM1-and IKZF3-targeting: A novel approach to multiple myeloma therapy synergy between CARM1 inhibition and IMiDs. [Abstract]2025 Feb 20;33(1):200952. PMID: 40123976 -
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J Med Chem
Discovery of ZLC491 as a Potent, Selective, and Orally Bioavailable CDK12/13 PROTAC Degrader. [Abstract]2024 Oct 24;67(20):18247-18264. PMID: 39388374 -
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Eur J Pharmacol
Doxorubicin-mediated retardation of aggresome formation enhances Carfilzomib-induced cell death synergistically by augmenting ER stress and proapoptotic signaling. [Abstract]2025 Dec 5:1008:178303. PMID: 41183585 -
Int Immunopharmacol
Pomalidomide promotes macrophage control of Mycobacterium tuberculosis via enhancing HDAC6-mediated autophagy. [Abstract]2025 Jun 17:158:114831. PMID: 40373598 -
Cancers (Basel)
Synergy between BRD9- and IKZF3-Targeting as a Therapeutic Strategy for Multiple Myeloma. [Abstract]2024 Mar 28;16(7):1319. PMID: 38610997 -
Structure
CAND1 and CAND2 drive CUL4 substrate receptor exchange with largely comparable biochemical efficiency, unlike their relative effects on CUL1. [Abstract]2026 Mar 20:S0969-2126(26)00056-0. PMID: 41864201 -
iScience
iTAG an optimized IMiD-induced degron for targeted protein degradation in human and murine cells. [Abstract]2023 Jun 7;26(7):107059. PMID: 37360684 -
Clin Exp Immunol
Bone marrow-derived mesenchymal stem cells inhibit CD8+ T cell immune responses via PD-1/PD-L1 pathway in multiple myeloma. [Abstract]2021 Jul;205(1):53-62. PMID: 33735518 -
Gen Comp Endocrinol
Tumour necrosis factor-α inhibits hepatic lipid deposition through GSK-3β/β-catenin signaling in juvenile turbot (Scophthalmus maximus L.). [Abstract]2016 Mar 1:228:1-8. PMID: 26747182
Pomalidomide purchased from MedChemExpress. Usage Cited in: Gen Comp Endocrinol. 2016 Mar 1:228:1-8. [Abstract]
TNFα significantly inhibits the mRNA and protein expression of GSK-3β, while POM significantly induces the mRNA and protein expression of GSK-3β (A). The mRNA and protein expression of β-catenin is significantly induced by TNFα, but significantly inhibited by POM (B). The protein expression of PPARγ and C/EBPα is significantly inhibited by TNFα, while significantly induced by POM treatment (C and D).
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Stem Cells Dev
The Supportive Role of Lymph Node Mesenchymal Stromal Cells in Follicular Lymphoma Involves the PITX1-hTERT-Podoplanin Axis. [Abstract]2025 Mar 25. PMID: 40130551 -
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bioRxiv
2026 May 21:2026.05.19.726384. PMID: 42239119 -
bioRxiv
Regulation of BCL11A DNA binding and expression in human erythrocyte precursor HUDEP-2 cells. [Abstract]2026 Feb 8:2026.02.06.704516. PMID: 41822826 -
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Elife
Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome. [Abstract]2018 Aug 1:7:e38430. PMID: 30067223
Pomalidomide purchased from MedChemExpress. Usage Cited in: Elife. 2018 Aug 1:7:e38430. [Abstract]
H9 hESC are treated with increasing concentrations of Thalidomide, Lenalidomide, Pomalidomide, or DMSO as a control. Following 24 h of incubation, SALL4 and GAPDH protein levels are assessed by western blot analysis.
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Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (182.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.15 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (301 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6598 mL | 18.2989 mL | 36.5979 mL | 91.4947 mL |
| 5 mM | 0.7320 mL | 3.6598 mL | 7.3196 mL | 18.2989 mL | |
| 10 mM | 0.3660 mL | 1.8299 mL | 3.6598 mL | 9.1495 mL | |
| 15 mM | 0.2440 mL | 1.2199 mL | 2.4399 mL | 6.0996 mL | |
| 20 mM | 0.1830 mL | 0.9149 mL | 1.8299 mL | 4.5747 mL | |
| 25 mM | 0.1464 mL | 0.7320 mL | 1.4639 mL | 3.6598 mL | |
| 30 mM | 0.1220 mL | 0.6100 mL | 1.2199 mL | 3.0498 mL | |
| 40 mM | 0.0915 mL | 0.4575 mL | 0.9149 mL | 2.2874 mL | |
| 50 mM | 0.0732 mL | 0.3660 mL | 0.7320 mL | 1.8299 mL | |
| 60 mM | 0.0610 mL | 0.3050 mL | 0.6100 mL | 1.5249 mL | |
| 80 mM | 0.0457 mL | 0.2287 mL | 0.4575 mL | 1.1437 mL | |
| 100 mM | 0.0366 mL | 0.1830 mL | 0.3660 mL | 0.9149 mL |
Keywords
- Pomalidomide
- 19171-19-8
- CC-4047
- Actimid
- CC4047
- CC 4047
- TNF Receptor
- Interleukin Related
- FOXP
- Orexin Receptor (OX Receptor)
- CDK
- Ligands for E3 Ligase
- Tumor Necrosis Factor Receptor
- TNFR
- IL
- Forkhead Box P Protein
- Hypocretin Receptor
- HCRT Receptor
- Cyclin dependent kinase
- E3 ligase-recruiting Moiety
- FOXP3
- IL-1
- IL-10
- IL-11
- IL-12
- IL-2
- IL-5
- T cells
- T-regulatory cell
- TNF-alpha
- Inhibitor
- inhibitor
- inhibit