Damulin B
Based on 1 publication(s) in Google Scholar
Damulin B is a dammarane-type saponin found in Gynostemma pentaphyllum. Damulin B can inhibit cancer cell apoptosis, decrease mitochondrial membrane potential, inhibit ROS production and cause G0/G1 phase arrest. Damulin B can prevent Cisplatin (HY-17394)-induced acute kidney injury and induce hair growth. Damulin B shows anti-inflammation anti-diabetic and anti-obesity effect. Damulin B can be used for the researches of cancer, inflammation, metabolic disease, such as lung cancer, osteoarthritis and diabetes.
For research use only. We do not sell to patients.
- Purity: 96.62%
- CAS No.: 1202868-75-4
- Formula: C42H70O13
- Molecular Weight:783.00
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Damulin B
More
Biological Activity
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CDK4 |
CDK6 |
Bcl-2 |
MMP-2 |
MMP-9 |
IL-24 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
23.8 μg/mL
Compound: Damulin B
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Cytotoxicity against human A549 cells assessed as decrease in viable cells after 48 hrs by CCK-8 assay
Cytotoxicity against human A549 cells assessed as decrease in viable cells after 48 hrs by CCK-8 assay
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[PMID: 26922140] |
Damulin B inhibits A549 and H1299 cells growth with IC50 values of 21.9 and 21.7 μM[1].
Damulin B (20-24 μM, 24 h) induces apoptosis and ROS production in A549 and H1299 cells[1].
Damulin B (20-24 μM, 24 h) cause the loss of mitochondrial membrane potential in A549 and H1299 cells[1].
Damulin B (20-24 μM, 24 h) reduces colony formation and inhibits migration in A549 and H1299 cells[1].
Damulin B (20-24 μM, 24 h) induces G0/G1 phase arrest in A549 and H1299 cells[1].
Damulin B (2.5-20 μM, 24 h) inhibits apoptosis in Cisplatin (HY-17394)-induced HEK293 cells[2].
Damulin B (0-80 μM, 24 h) shows no effect on cells viability in SW1353 cells[4].
Damulin B (10-80 μM, 1 h) inhibits IL-1β-induced NO and PGE2 production in SW1353 cells[4].
Damulin B (3-12 μM) inhibit cartilage-degrading enzymes in IL-1β-induced SW1353 cells[4].
Damulin B (1.2-12 μM) increases 2-deoxy-[3H]D-glucose uptake in L6 myotube cells[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 and H1299 cells
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Concentration:20 and 24 μM
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Incubation Time:24 h
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Result:Reduced Bcl-2, CDK4, CDK6, cyclin D1, cyclin E1 MMP-2 and MMMP-9 levels.
Increased IL-24, Bax, Bid, tBid and P53 levels.
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Cell Line:A549 and H1299 cells
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Concentration:20 and 24 μM
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Incubation Time:24 h
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Result:Increased cells in G0/G1 phase and reduced cells in S phase and G2/M phase.
Damulin B (0.9 mL/day, p.o.) induce hair growth via the Wnt/β-catenin pathway through AKT signaling in mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Cisplatin (HY-17394)-induced acute kidney injury mice models[1]
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Dosage:25 and 50 mg/kg
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Administration:Intraperitoneally injection, daily for 7 days
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Result:Increased AMPKα1 levels.
Prevented weight loss.
Attenuated tubular damage.
Chemical Information
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CAS No. 1202868-75-4
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Appearance Solid
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Molecular Weight 783.00
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Formula C42H70O13
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Color White to off-white
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SMILES
O[C@@H]([C@@H]([C@@H](CO)O1)O)[C@@H](O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)[C@@H]1O[C@H]3[C@H](O)C[C@]4(C)[C@@]5([H])C[C@@H](O)[C@@H]6[C@@H](C(CC/C=C(C)\C)=C)CC[C@@]6(C)[C@@](C)5CC[C@@]4([H])C3(C)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Int J Mol Sci
Inhibitory Effects of Heat-Processed Gynostemma pentaphyllum Extract (Actiponin®) and Its Components on Cartilage Breakdown in Osteoarthritis. [Abstract]2025 Feb 18;26(4):1728. PMID: 40004191
Solvent & Solubility
DMSO : ≥ 50 mg/mL (63.86 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (1.60 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1.25 mg/mL (1.60 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Shao-Fang Xing, et al. Inhibitory Effect of Damulin B from Gynostemma pentaphyllum on Human Lung Cancer Cells. Planta Med. 2019 Mar;85(5):394-405. [Content Brief]
[2]. Song M, et al. A Hydrodistillate of Gynostemma pentaphyllum and Damulin B Prevent Cisplatin-Induced Nephrotoxicity In Vitro and In Vivo via Regulation of AMPKα1 Transcription. Nutrients. 2022 Nov 24;14(23):4997. [Content Brief]
[3]. Kovale L, et al. Gynostemma pentaphyllum Hydrodistillate and Its Major Component Damulin B Promote Hair Growth-Inducing Properties In Vivo and In Vitro via the Wnt/β-Catenin Pathway in Dermal Papilla Cells. Nutrients. 2024 Mar 28;16(7):985. [Content Brief]
[4]. Lee SA, et al. Inhibitory Effects of Heat-Processed Gynostemma pentaphyllum Extract (Actiponin®) and Its Components on Cartilage Breakdown in Osteoarthritis. Int J Mol Sci. 2025 Feb 18;26(4):1728. [Content Brief]
[5]. Xu S, et al. Metabolomics and molecular docking-directed anti-obesity study of the ethanol extract from Gynostemma pentaphyllum (Thunb.) Makino. J Ethnopharmacol. 2024 Nov 15;334:118577. [Content Brief]
[6]. Nguyen PH, et al. New dammarane-type glucosides as potential activators of AMP-activated protein kinase (AMPK) from Gynostemma pentaphyllum. Bioorg Med Chem. 2011 Nov 1;19(21):6254-60. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2771 mL | 6.3857 mL | 12.7714 mL | 31.9285 mL |
| 5 mM | 0.2554 mL | 1.2771 mL | 2.5543 mL | 6.3857 mL | |
| 10 mM | 0.1277 mL | 0.6386 mL | 1.2771 mL | 3.1928 mL | |
| 15 mM | 0.0851 mL | 0.4257 mL | 0.8514 mL | 2.1286 mL | |
| 20 mM | 0.0639 mL | 0.3193 mL | 0.6386 mL | 1.5964 mL | |
| 25 mM | 0.0511 mL | 0.2554 mL | 0.5109 mL | 1.2771 mL | |
| 30 mM | 0.0426 mL | 0.2129 mL | 0.4257 mL | 1.0643 mL | |
| 40 mM | 0.0319 mL | 0.1596 mL | 0.3193 mL | 0.7982 mL | |
| 50 mM | 0.0255 mL | 0.1277 mL | 0.2554 mL | 0.6386 mL | |
| 60 mM | 0.0213 mL | 0.1064 mL | 0.2129 mL | 0.5321 mL |
Damulin B Related Classifications
HY-16942 Related Classifications
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