Cat. No.: HY-N0826

Corynoline

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Corynoline is an orally active acetylcholinesterase (AChE) inhibitor with an IC₅₀ of 30.6 μM. Corynoline exhibits multiple activities including anti-inflammatory, antinociceptive, antitumor and analgesic effects. Corynoline can be used in the research of diseases such as tumors and inflammatory pain.

For research use only. We do not sell to patients.

Purity: 98.84%
CAS No.: 18797-79-0
Formula: C₂₁H₂₁NO₅
Molecular Weight:367.40

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Corynoline Chemical Structure

CAS No.: 18797-79-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]^[3]

AChE

HO-1

IL-1β

Caspase 3

Caspase 9

p-STAT3

Bcl-2

COX-2

All Cholinesterase (ChE) Isoforms :

AChE
BChE

All Heme Oxygenase (HO) Isoforms :

HO-1
HO-2

All COX Isoforms :

All NO Synthase Isoforms :

All p38 MAPK Isoforms :

All JNK Isoforms :

All Bcl-2 Family Isoforms :

All Caspase Isoforms :

All STAT Isoforms :

All Interleukin Related Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	2.51 μM Compound: 6	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS induced nitric oxide production preincubated for 2 hrs followed by LPS challenge measured after 24 hrs by Griess reagent based assay Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS induced nitric oxide production preincubated for 2 hrs followed by LPS challenge measured after 24 hrs by Griess reagent based assay	[PMID: 29853329]

In Vitro

Corynoline (1-4 μM; 24 h) inhibits LPS (HY-D1056)-induced NO production in RAW264.7 cells, upregulates the levels of Nrf2, HO-1 and NQO1, and downregulates the expression of iNOS and COX-2^[1].
Corynoline (1-4 μM; 24 h) downregulates LPS-induced IL-1β and TNF-α expression at both mRNA and protein levels in RAW264.7 cells in a dose-dependent manner^[1].
Corynoline (1 μM; 24 h) inhibits LPS-induced phosphorylation of p38 and JNK in RAW264.7 cells^[1].
Corynoline (5-40 μM; 24-48 h) inhibits the proliferation and induces apoptosis of U87 and LN229 glioblastoma cells^[2].
Corynoline (10-20 μM; 10-14 days) inhibits the colony-forming ability of U87 glioblastoma cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

RAW264.7 treated LPS (HY-D1056)
Concentration:

1, 2, 4 μM
Incubation Time:

24 h
Result:

Upregulated the levels of Nrf2, HO-1 and NQO1, and downregulated the levels of iNOS and COX-2.

Cell Proliferation Assay^[2]

Cell Line:

U87 glioblastoma cell line, LN229 glioblastoma cell line, human astrocyte (HA) cell line
Concentration:

5, 10, 20, 40 μM
Incubation Time:

24 h, 48 h
Result:

Inhibited the viability of U87 and LN229 cells in a dose-dependent manner. Exhibited no significant toxicity towards HA cells.

Immunofluorescence^[2]

Cell Line:

U87 glioblastoma cell line, LN229 glioblastoma cell line
Concentration:

10, 20 μM
Incubation Time:

48 h
Result:

Significantly reduced the percentage of Ki-67-positive cells in both U87 and LN229 cells. Reduced the proportion of Ki-67-positive cells to less than 50% at 20 μM.

Western Blot Analysis^[2]

Cell Line:

U87 glioblastoma cell line, LN229 glioblastoma cell line
Concentration:

10, 20 μM
Incubation Time:

48 h
Result:

Significantly increased the protein levels of cleaved caspase-9 and cleaved caspase-3 in both U87 and LN229 cells.
Inhibited STAT3 phosphorylation and Bcl-2 expression in a concentration-dependent manner in both U87 and LN229 cells. Upregulated the pro-apoptotic proteins Bak and Bax.

In Vivo

Corynoline (50 mg/kg; i.p.; 21 days) exerts antitumor activity in mouse models of glioblastoma^[2].
Corynoline (5-20 mg/kg; oral administration; single dose) exerts a significant dose-dependent antinociceptive and anti-inflammatory effect in various nociceptive and inflammatory models of male mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c nude (6 weeks old, 20-22 g, subcutaneously injected with GBM cells)^[2]
Dosage:

50 mg/kg
Administration:

i.p.; daily; 21 days
Result:

Significantly reduced tumor volume.
Decreased the percentage of Ki-67-positive cells; downregulated phosphorylated STAT3 and Bcl-2 protein levels.
Upregulated Bak, Bax, cleaved caspase-9, and cleaved caspase-3 protein levels in tumor tissues.

Animal Model:

Swiss mice (male, 20-30 g)^[3]
Dosage:

5, 10, 20 mg/kg
Administration:

p.o.; single dose
Result:

Significantly increased the latency time of pain response in hot plate and tail immersion tests in a dose-dependent manner.
Reduced the number of abdominal writhing in acetic acid-induced model, the paw licking count in glutamate, capsaicin and formalin-induced models, and inhibited both the neurogenic phase and inflammatory phase of formalin-induced nociception.
Significantly suppressed carrageenan-induced paw edema, reduced the infiltration of leukocytes, mononuclear cells and polymorphonuclear cells in the peritoneal cavity induced by carrageenan, and down-regulated the levels of pro-inflammatory cytokines TNF-α, IL-1β and IL-6 in the air pouch model.

Chemical Information

CAS No. 18797-79-0
Appearance Solid
Molecular Weight 367.40
Formula C₂₁H₂₁NO₅
Color White to light yellow
SMILES

C[C@@]1(C(C=CC2=C3OCO2)=C3CN4C)[C@@]4([H])C(C=C(OCO5)C5=C6)=C6C[C@@H]1O
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (6)

All Histological Imaging/StainingWBIHCRT-PCRIF

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Journal Impact Factor

Biomed Pharmacother

Corynoline protects ang II-induced hypertensive heart failure by increasing PPARα and Inhibiting NF-κB pathway. [Abstract]

2022 Jun:150:113075. PMID: 35658238

Corynoline purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2022 Jun:150:113075. [Abstract]

Representative micrographs of Masson Trichromestaining of interstitial fibrotic areas (%) from Masson Trichrome staining treated with Corynoline (COR) (20 mg/kg, i.g.).

Corynoline purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2022 Jun:150:113075. [Abstract]

Western blot analysis of β-MyHC, COL-1, and TGF-β1 in heart tissues treated with Corynoline (COR) (20 mg/kg, i.g.).

Corynoline purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2022 Jun:150:113075. [Abstract]

The heart was harvested to perform immunohistochemical staining for macrophage marker (F4/80) of F4/80-positive cells treated with Corynoline (COR) (20 mg/kg, i.g.).

Corynoline purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2022 Jun:150:113075. [Abstract]

The mRNA levels of myh7, Col1a, and Tgfb1 were detected by RT-qPCR in the heart tissues treated with Corynoline (COR) (20 mg/kg, i.g.).

Corynoline purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2022 Jun:150:113075. [Abstract]

TRITC phalloidin (red) staining of H9c2 cells showing the effect of Corynoline (COR) (5, 10 μM) on Ang II-induced hypertrophic responses.
J Gastroenterol

Corynoline protects chronic pancreatitis via binding to PSMA2 and alleviating pancreatic fibrosis. [Abstract]

2024 Nov;59(11):1037-1051. PMID: 39145797
Inflamm Res

Corynoline alleviates hepatic ischemia-reperfusion injury by inhibiting NLRP3 inflammasome activation through enhancing Nrf2/HO-1 signaling. [Abstract]

2024 Dec;73(12):2069-2085. PMID: 39294398
Antiviral Res

Discovery of hepatitis B virus subviral particle biogenesis inhibitors from a bioactive compound library. [Abstract]

2024 Jul 2:228:105955. PMID: 38964614
Naunyn Schmiedebergs Arch Pharmacol

Corynoline promotes apoptosis and inhibits proliferation of glioblastoma via regulating the STAT3/Bcl-2 signalling pathway. [Abstract]

2025 Dec 11. PMID: 41379317
Immunopharmacol Immunotoxicol

Corynoline ameliorates dextran sulfate sodium-induced colitis in mice by modulating Nrf2/NF-κB pathway. [Abstract]

2023 Feb;45(1):26-34. PMID: 35980837

Solvent & Solubility

In Vitro:

DMSO : 40 mg/mL (108.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7218 mL

13.6091 mL

27.2183 mL

5 mM

0.5444 mL

2.7218 mL

5.4437 mL

10 mM

0.2722 mL

1.3609 mL

2.7218 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 4 mg/mL (10.89 mM); Clear solution

This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (287 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kim DK. Inhibitory effect of corynoline isolated from the aerial parts of Corydalis incisa on the acetylcholinesterase. Arch Pharm Res. 2002 Dec;25(6):817-9. [Content Brief]

[2]. Yang C, et al. Corynoline Isolated from Corydalis bungeana Turcz. Exhibits Anti-Inflammatory Effects via Modulation of Nfr2 and MAPKs. Molecules. 2016;21(8):975. Published 2016 Jul 27. [Content Brief]

[3]. Xiong Y, et al. Corynoline promotes apoptosis and inhibits proliferation of glioblastoma via regulating the STAT3/Bcl-2 signalling pathway. Naunyn Schmiedebergs Arch Pharmacol. Published online December 11, 2025. [Content Brief]

[4]. Lei F, et al. Antinociceptive and Anti-inflammatory Effect of Corynoline in Different Nociceptive and Inflammatory Experimental Models. Appl Biochem Biotechnol. 2022;194(10):4783-4799. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7218 mL	13.6091 mL	27.2183 mL	68.0457 mL
	5 mM	0.5444 mL	2.7218 mL	5.4437 mL	13.6091 mL
	10 mM	0.2722 mL	1.3609 mL	2.7218 mL	6.8046 mL
	15 mM	0.1815 mL	0.9073 mL	1.8146 mL	4.5364 mL
	20 mM	0.1361 mL	0.6805 mL	1.3609 mL	3.4023 mL
	25 mM	0.1089 mL	0.5444 mL	1.0887 mL	2.7218 mL
	30 mM	0.0907 mL	0.4536 mL	0.9073 mL	2.2682 mL
	40 mM	0.0680 mL	0.3402 mL	0.6805 mL	1.7011 mL
	50 mM	0.0544 mL	0.2722 mL	0.5444 mL	1.3609 mL
	60 mM	0.0454 mL	0.2268 mL	0.4536 mL	1.1341 mL
	80 mM	0.0340 mL	0.1701 mL	0.3402 mL	0.8506 mL
	100 mM	0.0272 mL	0.1361 mL	0.2722 mL	0.6805 mL

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Corynoline

Apoptosis Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

MAPK Compound Library

Metabolism/Protease Compound Library

Neuronal Signaling Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

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Anti-Pulmonary Fibrosis Compound Library

Pain-Related Compound Library

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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

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Publications Citing Use of MedChemExpress (MCE) Corynoline

All Cholinesterase (ChE) Isoforms

All Heme Oxygenase (HO) Isoforms

All COX Isoforms

All NO Synthase Isoforms

All p38 MAPK Isoforms

All JNK Isoforms

All Bcl-2 Family Isoforms

All Caspase Isoforms

All STAT Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation