Dihydrocapsaicin
Based on 5 publication(s) in Google Scholar
Dihydrocapsaicin, a capsaicin, is a potent and selective TRPV1 (transient receptor potential vanilloid channel 1) agonist. Dihydrocapsaicin reduces AIF, Bax, and Caspase-3 expressions, and increased Bcl-2, Bcl-xL and p-Akt levels. Dihydrocapsaicin enhances the hypothermia-induced neuroprotection following ischemic stroke via PI3K/Akt regulation in rat.
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- Purity : 99.93%
- CAS No.: 19408-84-5
- 화학식: C18H29NO3
- 분자량:307.43
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Dihydrocapsaicin
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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Apoptosis Analysis
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WB
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IF
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Histological Imaging/Staining
All Caspase Isoforms
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Biological Activity
제품 설명
IC50 & Target
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TRPV1 |
PI3K |
Caspase 3 |
Bax |
Bcl-2 |
Bcl-xL |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Astrocyte | EC50 |
41.83 μM
Compound: 15
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Antiproliferative activity against mouse astrocyte cells by MTT assay
Antiproliferative activity against mouse astrocyte cells by MTT assay
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[PMID: 17417631] |
| HeLa | IC50 |
>50 μM
Compound: Dihydrocapsaicin
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Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
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[PMID: 29685681] |
| K562 | IC50 |
>50 μM
Compound: Dihydrocapsaicin
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Antiproliferative activity against human K562 cells after 48 hrs by MTT assay
Antiproliferative activity against human K562 cells after 48 hrs by MTT assay
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[PMID: 29685681] |
| MCF7 | IC50 |
>50 μM
Compound: Dihydrocapsaicin
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Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
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[PMID: 29685681] |
| Medulloblastoma cell | EC50 |
0.02 μM
Compound: 15
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Antiproliferative activity against mouse medulloblastoma cells harboring heterozygous ptch1 gene by MTT assay
Antiproliferative activity against mouse medulloblastoma cells harboring heterozygous ptch1 gene by MTT assay
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[PMID: 17417631] |
In Vitro
Dihydrocapsaicin (0-100 μM) inhibits platelet aggregation and the activity of clotting factors VIII:C (6.26-100 μM) and IX (25-100 μM)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley rats (adult, male, 300-340 g, subjected to right middle cerebral artery occlusion (MCAO)[2]
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Dosage:0.5 mg/kg
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Administration:IP, once
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Result:Exhibits hypothermic effect, rectal temperature dropped to approximately 35.0 ℃ at 30 min, stayed at equal or below 35.0 ℃ for approximately 20 min, and then gradually returned to approximately 36.5 ℃ at 120 min. Significantly reduced Ischemia-reperfusion induced infarct volume (36.2% ± 2.5%). Reduces ROS levels at 24 h, and reduced ischemia-reperfusion induced a high level of cell death.
Chemical Information
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CAS No. 19408-84-5
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Appearance Solid
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분자량 307.43
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화학식 C18H29NO3
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Color White to off-white
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SMILES
CC(C)CCCCCCC(NCC1=CC=C(O)C(OC)=C1)=O
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (5)
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Journal Impact Factor
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Most Recent
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Phytother Res
Dihydrocapsaicin suppresses the STING-mediated accumulation of ROS and NLRP3 inflammasome and alleviates apoptosis after ischemia-reperfusion injury of perforator skin flap. [Abstract]2024 May;38(5):2539-2559. PMID: 38459660
Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2024 May;38(5):2539-2559. [Abstract]
Immunofluorescence staining for CASP3 (red) and CD31 (green) in the skin tissue of control and Dihydrocapsaicin (DHC) (5 mg/kg, p.o) groups.
Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2024 May;38(5):2539-2559. [Abstract]
Typical HE staining of skin tissues in control and Dihydrocapsaicin (DHC) (5 mg/kg,p.o.) groups.
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Cancer Manag Res
Dihydrocapsaicin Secreted by RYK Silenced Bone Marrow-Derived Mesenchymal Stem Cells Triggers Apoptosis of Gastric Cancer Cells. [Abstract]2026 Mar 21:18:579598. PMID: 41890221
Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Cancer Manag Res. 2026 Mar 21:18:579598. [Abstract]
The proliferation of NCI-N87 cells was detected by CCK-8 at 12, 24, and 48 h treated with Dihydrocapsaicin (DHC) (20, 40, 60, 80 μM).
Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Cancer Manag Res. 2026 Mar 21:18:579598. [Abstract]
Effect of Dihydrocapsaicin (DHC) (60, 80 μM) on apoptosis of NCI-N87 cells. The apoptosis of NCI-N87 cells was detected by flow cytometry.
Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Cancer Manag Res. 2026 Mar 21:18:579598. [Abstract]
The apoptosis-related protein expressions of Bax, Caspase3, and Caspase9 in NCI-N87 cells were detected by WB treated with Dihydrocapsaicin (DHC) (60, 80 μM).
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용액&용해도
In Vitro:
DMSO : 100 mg/mL (325.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
순도&문서
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Data Sheet (272 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Gao F, et al. Impairment in function and expression of transient receptor potential vanilloid type 4 in Dahl salt-sensitive rats: significance and mechanism. Hypertension. 2010 Apr;55(4):1018-25. [Content Brief]
[2]. Dihydrocapsaicin, et al. Dihydrocapsaicin (DHC) enhances the hypothermia-induced neuroprotection following ischemic stroke via PI3K/Akt regulation in rat. Brain Res. 2017 Sep 15;1671:18-25. [Content Brief]
[3]. Adams MJ, et al. Effect of capsaicin and dihydrocapsaicin on in vitro blood coagulation and platelet aggregation. Thromb Res. 2009 Dec;124(6):721-3. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2528 mL | 16.2639 mL | 32.5277 mL | 81.3193 mL |
| 5 mM | 0.6506 mL | 3.2528 mL | 6.5055 mL | 16.2639 mL | |
| 10 mM | 0.3253 mL | 1.6264 mL | 3.2528 mL | 8.1319 mL | |
| 15 mM | 0.2169 mL | 1.0843 mL | 2.1685 mL | 5.4213 mL | |
| 20 mM | 0.1626 mL | 0.8132 mL | 1.6264 mL | 4.0660 mL | |
| 25 mM | 0.1301 mL | 0.6506 mL | 1.3011 mL | 3.2528 mL | |
| 30 mM | 0.1084 mL | 0.5421 mL | 1.0843 mL | 2.7106 mL | |
| 40 mM | 0.0813 mL | 0.4066 mL | 0.8132 mL | 2.0330 mL | |
| 50 mM | 0.0651 mL | 0.3253 mL | 0.6506 mL | 1.6264 mL | |
| 60 mM | 0.0542 mL | 0.2711 mL | 0.5421 mL | 1.3553 mL | |
| 80 mM | 0.0407 mL | 0.2033 mL | 0.4066 mL | 1.0165 mL | |
| 100 mM | 0.0325 mL | 0.1626 mL | 0.3253 mL | 0.8132 mL |
Dihydrocapsaicin Related Classifications
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