Anticancer agent 306
Anticancer agent 306 is a spiro tetramic acid derivative and a inhibitor of MMP1, MMP7 and PLK1. Anticancer agent 306 exerts antiproliferative activity against H1299, RKO and MCF-7 cells with IC50 values of 19.25 μM, 3.29 μM and 102.36 μM, respectively. Anticancer agent 306 can up-regulate p21 protein and down-regulate CCND1 and CCNB1 proteins to induce cell cycle arrest, regulate the expression of Bcl-2 and Bax to induce cell apoptosis. Anticancer agent 306 can down-regulate MMP1 and MMP7 proteins to inhibit tumor invasion and metastasis. Anticancer agent 306 can be used for the research of lung cancer, colorectal cancer, breast cancer.
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- CAS No.: 3043892-01-6
- Formule: C19H21Cl2NO3
- Masse moléculaire:382.28
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Activité biologique
Description
IC50 & Target
[1]|
MMP-1 |
MMP-7 |
PLK1 |
Bcl-2 |
Bax |
Caspase 3 |
Caspase 8 |
In Vitro
Anticancer agent 306 (8D) potently inhibits the proliferation of H1299, RKO, and MCF-7 cells with IC50 values of 19.25 μM, 3.29 μM, and 102.36 μM, respectively[1].
Anticancer agent 306 (10-40 μg/mL) dose-dependently down-regulates MMP1, MMP7, PLK1, CCND1, and Bcl-2, and up-regulates p21, Bax, caspase-3, and caspase-8 in H1299 and RKO cells[1].
Anticancer agent 306 (10-40 μg/mL) induces G1-phase cell cycle arrest in H1299 and RKO cells, with a corresponding decrease in S and G2 phase cell proportions[1].
Anticancer agent 306 (10-40 μg/mL) dose-dependently induces early and late apoptosis in H1299 and RKO cells, via the mitochondrial pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- RNA extraction experimental
- Ex Vivo Tissue Slice/Explant Invasion Assay
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Protocol for Cell Cycle
- Breast Cancer Modeling
- 3D Tumor Spheroid Invasion Assay
- Cell Viability Determination by MTT Colorimetric Assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Apoptosis Solutions
- Organotypic 3D Invasion Assay
- Cell invasion
- Orthotopic Cell-Line Xenograft
Chemical Information
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CAS No. 3043892-01-6
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Masse moléculaire 382.28
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Formule C19H21Cl2NO3
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SMILES
ClC1=CC(Cl)=C(C2=C(OC(CC(C)C)=O)C3(CCCC3)NC2=O)C=C1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anticancer agent 306 Related Classifications
HY-181769 Related Classifications
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