Cat. No.: HY-N0404

Sinigrin

(Synonyms: Allyl-glucosinolate; 2-Propenyl-glucosinolate)

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Sinigrin (Allyl-glucosinolate) is an orally active glucosinolate found in cruciferous plants. Sinigrin possesses multiple activities such as anti-cancer, antibacterial, antifungal, anti-inflammatory, antioxidant, and inhibition of fat synthesis. Sinigrin can be used in the research of tumors, inflammatory, and metabolic diseases.

For research use only. We do not sell to patients.

Purity: 99.92%
CAS No.: 3952-98-5
Formula: C₁₀H₁₆KNO₉S₂
Molecular Weight:397.46

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Sinigrin Chemical Structure

CAS No.: 3952-98-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]

PPARγ

IL-6

IL-1β

CDK4

CDK6

All p38 MAPK Isoforms :

All AMPK Isoforms :

All Interleukin Related Isoforms :

All PPAR Isoforms :

All CDK Isoforms :

All PI3K Isoforms :

All Akt Isoforms :

All mTOR Isoforms :

In Vitro

Sinigrin (1-100 μg/mL; 1-8 days) inhibits lipid accumulation, downregulates the expression of adipogenesis-related genes, suppresses the production of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β, IL-18), and induces cell cycle arrest in 3T3-L1 cells. The mechanism involves activation of the AMPK pathway and inhibition of the MAPK pathway^[1].
Sinigrin (5-30 μM; 24-48 h) induces cell cycle arrest in the S phase and promotes apoptosis, while triggering ROS-dependent oxidative stress damage by inhibiting PI3K/AKT/mTOR phosphorylation in MCF-7 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:

3T3-L1 adipocytes
Concentration:

100 μg/mL
Incubation Time:

24 h
Result:

Arrested cells in the G0/G1phase of the cell cycle and increased the expression of p21 and p27.

Western Blot Analysis^[1]

Cell Line:

3T3-L1 adipocytes
Concentration:

1, 10, and 100 μg/mL
Incubation Time:

8 days
Result:

Remarkably inhibited the accumulation of lipid droplets and adipogenesis by downregulating the expression of CCAAT-enhancer-binding protein α (C/EBPα), PPARγ, leptin and aP2.

RT-PCR^[1]

Cell Line:

3T3-L1 adipocytes
Concentration:

1, 10, and 100 μg/mL
Incubation Time:

8 days
Result:

Inhibited the production of pro-inflammatory cytokines including TNF-α and IL-6, IL-1β and IL-18.

Western Blot Analysis^[2]

Cell Line:

MCF-7 cells
Concentration:

20 μM
Incubation Time:

24 and 48 h
Result:

Inhibited the levels of cyclin-D1, PCNA, CDK4, and CDK6.

In Vivo

Sinigrin (15-30 mg/kg; oral administration; 12 days) exerts anti-inflammatory and ameliorative effects in DSS (HY-116282C)-induced ulcerative colitis mouse models^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male BALB/c mice (20-25 gm) treated DSS (HY-116282C)^[3]
Dosage:

15 and 30 mg/kg
Administration:

Oral administration; 12 days
Result:

Significantly mitigated the DSS-induced body weight loss, attenuated the colon length shrinkage, and improved the disease index score.
Successfully abrogated the DSS-induced IL-17 levels and improved the colonic barrier in colon tissues.

Chemical Information

CAS No. 3952-98-5
Appearance Solid
Molecular Weight 397.46
Formula C₁₀H₁₆KNO₉S₂
Color White to off-white
SMILES

O[C@@H]1[C@@H](O)[C@H](S/C(CC=C)=N/OS(=O)(O[K])=O)O[C@H](CO)[C@H]1O
Synonyms

Allyl-glucosinolate; 2-Propenyl-glucosinolate
Structure Classification
- Alkaloids
- Other Alkaloids
- Glucosinolates
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (4)

All Cell Proliferation/Viability AssayCell Imaging/StainingWB

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Journal Impact Factor

Food Chem X

Magnetic field-assisted blanching enhances sulforaphane synthesis in broccoli florets, revealed by UPLC-ESI-QTRAP-MS/MS. [Abstract]

2026 Feb 20:34:103689. PMID: 41777636
Phytother Res

Identification of Sinigrin as Active Compound of Rape Pollen for Treating Benign Prostatic Hyperplasia Through PI3K/AKT/mTOR Axis. [Abstract]

2025 May 8. PMID: 40343383

Sinigrin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 May 8. [Abstract]

Cell proliferation of BPH-1 cells after treating by Sinigrin (10, 50, 100 μM) for 48h.

Sinigrin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 May 8. [Abstract]

Cloning efficiency of BPH-1 treated with LPS and Sinigrin (50, 100 μM) for 7days.

Sinigrin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 May 8. [Abstract]

Western Blot analysis of PI3K/AKT/mTOR pathway in RWPE-1 and BPH-1 cells after treating with 50μM Sinigrin for 48h.
Lebensm Wiss Technol

2025 Dec 23.
Plant Physiol Biochem

Exploring the mechanism of blindness physiopathy in Brassica oleracea var italica L. by comprehensive transcriptomics and metabolomics analysis. [Abstract]

2024 Jan:206:108304. PMID: 38159550

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (62.90 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5160 mL

12.5799 mL

25.1598 mL

5 mM

0.5032 mL

2.5160 mL

5.0320 mL

10 mM

0.2516 mL

1.2580 mL

2.5160 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lee HW, et al. Inhibitory effect of sinigrin on adipocyte differentiation in 3T3-L1 cells: Involvement of AMPK and MAPK pathways. Biomed Pharmacother. 2018 Jun;102:670-680. [Content Brief]

[2]. Li S, et al. Sinigrin Impedes the Breast Cancer Cell Growth through the Inhibition of PI3K/AKT/mTOR Phosphorylation-Mediated Cell Cycle Arrest. J Environ Pathol Toxicol Oncol. 2022;41(3):33-43. [Content Brief]

[3]. Rama Satya Sri Kotipalli, et al. Sinigrin Attenuates the Dextran Sulfate Sodium-induced Colitis in Mice by Modulating the MAPK Pathway. Inflammation. 2023 Jun;46(3):787-807. [Content Brief]

[4]. Mazumder A, et al. Sinigrin and Its Therapeutic Benefits. Molecules. 2016 Mar 29;21(4):416. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	2.5160 mL	12.5799 mL	25.1598 mL	62.8994 mL
	5 mM	0.5032 mL	2.5160 mL	5.0320 mL	12.5799 mL
	10 mM	0.2516 mL	1.2580 mL	2.5160 mL	6.2899 mL
	15 mM	0.1677 mL	0.8387 mL	1.6773 mL	4.1933 mL
	20 mM	0.1258 mL	0.6290 mL	1.2580 mL	3.1450 mL
	25 mM	0.1006 mL	0.5032 mL	1.0064 mL	2.5160 mL
	30 mM	0.0839 mL	0.4193 mL	0.8387 mL	2.0966 mL
	40 mM	0.0629 mL	0.3145 mL	0.6290 mL	1.5725 mL
	50 mM	0.0503 mL	0.2516 mL	0.5032 mL	1.2580 mL
	60 mM	0.0419 mL	0.2097 mL	0.4193 mL	1.0483 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Sinigrin

Anti-Infection Compound Library

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Epigenetics Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Glycoside Compound Library

Oxygen Sensing Compound Library

Antifungal Compound Library

Antibacterial Compound Library

Medicine Food Homology Compound Library

Glycolysis Compound Library

Pyroptosis Compound Library

Cytoskeleton Compound Library

Orally Active Compound Library

Glutamine Metabolism Compound Library

Traditional Chinese Medicine Active Compound Library

Alkaloids Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Lipid Metabolism Compound Library

Glucose Metabolism Compound Library

Food-Sourced Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Cancer Natural Product Library

Antidepressant Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Nuclear Receptor Compound Library

Osteogenesis Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Metabolic Enzyme Compound Library

Membrane Protein-targeted Compound Library

Cytokine Inhibitors Library

Cell Death Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Anti-Brain Cancer Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library