2. MAPK/ERK Pathway PI3K/Akt/mTOR Epigenetics
  3. p38 MAPK AMPK
  4. Sinigrin

Sinigrin is a major glucosinolate present in plants of the Brassicaceae family. Sinigrin inhibits early-stage adipogenesis of 3T3-L1 adipocytes through the AMPK and MAPK signaling pathways. Sinigrin has potent anti-oxidant, anti-tumor and anti-inflammatory effects.

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Sinigrin Chemical Structure

Sinigrin Chemical Structure

CAS No. : 3952-98-5

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Based on 1 publication(s) in Google Scholar

Sinigrin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Sinigrin

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1

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Description

Sinigrin is a major glucosinolate present in plants of the Brassicaceae family. Sinigrin inhibits early-stage adipogenesis of 3T3-L1 adipocytes through the AMPK and MAPK signaling pathways. Sinigrin has potent anti-oxidant, anti-tumor and anti-inflammatory effects[1].

In Vitro

Sinigrin (100 μg/mL; 24 H) arrestsd cells in the G0/G1phase of the cell cycle and increased the expression of p21 and p27[1].
Sinigrin (1-100 μg/mL; 8 days) remarkably inhibits the accumulation of lipid droplets and adipogenesis by downregulating the expression of C/EBPα, PPARγ, leptin and aP2. Sinigrin increases the phosphorylation of AMPK, MAPK and acetyl-CoA carboxylase (ACC) in the early stage of adipocyte differentiation, suggesting that sinigrin has anti-adipogenic effects through AMPK, MAPK and ACC activation[1].
Sinigrin (1-100 μg/mL; 8 days) inhibits the production of pro-inflammatory cytokines including TNF-α and IL-6, IL-1β and IL-18[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sinigrin Related Antibodies

Cell Cycle Analysis[1]

Cell Line: 3T3-L1 adipocytes
Concentration: 100 μg/mL
Incubation Time: 24 h
Result: Arrested cells in the G0/G1phase of the cell cycle and increased the expression of p21 and p27.

Western Blot Analysis[1]

Cell Line: 3T3-L1 adipocytes
Concentration: 1, 10, and 100 μg/mL
Incubation Time: 8 days
Result: Remarkably inhibited the accumulation of lipid droplets and adipogenesis by downregulating the expression of CCAAT-enhancer-binding protein α (C/EBPα), PPARγ, leptin and aP2.

RT-PCR[1]

Cell Line: 3T3-L1 adipocytes
Concentration: 1, 10, and 100 μg/mL
Incubation Time: 8 days
Result: Inhibited the production of pro-inflammatory cytokines including TNF-α and IL-6, IL-1β and IL-18.
In Vivo

Sinigrin (15-30 mg/kg; Oral administration; for 12 days) exerts protective and therapeutic effects on DSS‑induced colitis, by enhancing the anti-oxidant enzymes and suppressing the intestinal inflammatory cascade of markers by regulating the MAPK pathway[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: A dextran sulfate sodium (DSS)-induced mouse model[2]
Dosage: 15 mg/kg or 30 mg/kg
Administration: Oral administration; for 12 days
Result: Significantly mitigated the DSS-induced body weight loss, attenuated the colon length shrinkage, and improved the disease index score. Successfully abrogated the DSS-induced IL-17 levels and improved the colonic barrier in colon tissues.
Molecular Weight

397.46

Formula

C10H16KNO9S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@@H](O)[C@H](S/C(CC=C)=N/OS(=O)(O[K])=O)O[C@H](CO)[C@H]1O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 12.5 mg/mL (31.45 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5160 mL 12.5799 mL 25.1598 mL
5 mM 0.5032 mL 2.5160 mL 5.0320 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Purity & Documentation

Purity: 99.97%

COA (254 KB) HNMR (273 KB) RP-HPLC (247 KB) MS (175 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.5160 mL 12.5799 mL 25.1598 mL 62.8994 mL
5 mM 0.5032 mL 2.5160 mL 5.0320 mL 12.5799 mL
10 mM 0.2516 mL 1.2580 mL 2.5160 mL 6.2899 mL
15 mM 0.1677 mL 0.8387 mL 1.6773 mL 4.1933 mL
20 mM 0.1258 mL 0.6290 mL 1.2580 mL 3.1450 mL
25 mM 0.1006 mL 0.5032 mL 1.0064 mL 2.5160 mL
30 mM 0.0839 mL 0.4193 mL 0.8387 mL 2.0966 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Sinigrin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sinigrin3952-98-5p38 MAPKAMPKAMP-activated protein kinaseadipogenesisadipocytedifferentiationInhibitorinhibitorinhibit

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