Cat. No.: HY-123364

Sargachromanol E

Sargachromanol E is an apoptosis inducer, growth inhibitor, anti-inflammatory agent, and anti-aging agent. Sargachromanol E activates caspase-3, mediates PARP cleavage, downregulates Bcl-xL and upregulates Bax levels, and promotes cell cycle arrest at the sub-G1 phase. It inhibits LPS-induced transcription and expression of COX-2 and iNOS (NO: IC₅₀ = 6.99 μg/mL), reduces the phosphorylation levels of p38 MAPK and ERK1/2, and suppresses the release of pro-inflammatory mediators including TNF‑α, IL‑1β, and prostaglandin E₂. Sargachromanol E can be used in research related to leukemia, oral squamous cell carcinoma, and inflammation.

For research use only. We do not sell to patients.

CAS No.: 856414-54-5
Formula: C₂₇H₄₀O₄
Molecular Weight:428.60

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Sargachromanol E Chemical Structure

CAS No.: 856414-54-5

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target^[2]^[4]^[1]

iNOS

6.99 μg/mL (IC₅₀)

Caspase-3

Bcl-xL

Bax

COX-2

p38 MAPK

ERK1

ERK2

IL-1β

All Caspase Isoforms :

All PARP Isoforms :

All Bcl-2 Family Isoforms :

All COX Isoforms :

All NO Synthase Isoforms :

All TNF Receptor Isoforms :

All Interleukin Related Isoforms :

All ERK Isoforms :

All p38 MAPK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	16.3 μM	Inhibition of lipopolysaccharide-induced nitric oxide production in murine RAW 264.7 macrophages. Inhibition of lipopolysaccharide-induced nitric oxide production in murine RAW 264.7 macrophages.	35539527
RAW264.7	IC₅₀	6.99 μg/mL	Inhibition of nitric oxide (NO) production in LPS-stimulated murine RAW 264.7 macrophages, assessed via Griess reaction after 2 h pretreatment with sargachromanol E followed by 24 h LPS incubation. Inhibition of nitric oxide (NO) production in LPS-stimulated murine RAW 264.7 macrophages, assessed via Griess reaction after 2 h pretreatment with sargachromanol E followed by 24 h LPS incubation.	23973192

In Vitro

Sargachromanol E (SE) (12.5-50 μM; 24 h) potently inhibits the proliferation of human promyelocytic leukemia HL-60 cells in vitro in a dose-dependent manner, with inhibition rates of 10.7%, 68.9% and 90.1% at concentrations of 12.5, 25 and 50 μM, respectively, and exhibits no cytotoxicity against normal cell lines at these concentrations^[1].
Sargachromanol E (12.5-50 μM; 24 h) induces nuclear pyknosis and fragmentation in human promyelocytic leukemia HL-60 cells^[1].
Sargachromanol E (12.5-50 μM; 24 h) induces apoptotic DNA fragmentation in human promyelocytic leukemia HL-60 cells^[1].
Sargachromanol E (12.5-25 μM; 24 h) regulates the expression of apoptosis-related proteins in human promyelocytic leukemia HL-60 cells; at concentrations of 12.5 μM and 25 μM, slight upregulation of Bax, significant downregulation of Bcl-xL, activation of caspase-3 and caspase-9, and cleavage of PARP are observed^[1].
Apoptosis induced by Sargachromanol E (25 μM; 24 h) in human promyelocytic leukemia HL-60 cells is attenuated by the caspase-3 inhibitor Z-DEVD-fmk (HY-12466)^[1].
Sargachromanol E (12.5-50 μM; 24 h) induces a concentration-dependent increase in apoptotic sub-G₁ hypodiploid cells without affecting the progression of the G₂/M phase in human promyelocytic leukemia HL-60 cells^[1].
Sargachromanol E (5-25 μg/mL; pre-treatment for 2 h) potently inhibits NO production in LPS-stimulated RAW 264.7 macrophages, with an IC₅₀ of 6.99 μg/mL, and shows no cytotoxicity even at concentrations as high as 25 μg/mL^[2].
Sargachromanol E (16.3 μM) inhibits NO production in LPS-stimulated mouse RAW 264.7 macrophages, with an IC₅₀ value of 16.3 μM^[4].
Sargachromanol E (5-25 μg/mL; pre-treatment for 2 h) dose-dependently inhibits the production of PGE₂, TNF-α, and IL-1β, reduces the protein expression of COX-2 and iNOS, suppresses the phosphorylation of ERK1/2 and JNK, and mildly decreases the phosphorylation of p38 in LPS-stimulated RAW 264.7 macrophages^[2].
Sargachromanol E induces caspase-3-mediated apoptosis in promyelocytic leukemia HL-60 cells^[4].
Sargachromanol E inhibits long-wave ultraviolet-induced senescence in human skin fibroblasts^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

human promyelocytic leukemia HL-60 cells
Concentration:

12.5, 25, 50 μM
Incubation Time:

24 h
Result:

Induced dose-dependent nuclear morphological changes characteristic of apoptosis.\n
Induced the characteristic DNA laddering pattern indicative of apoptotic DNA fragmentation in treated cells.

Cell Cycle Analysis^[1]

Cell Line:

human promyelocytic leukemia HL-60 cells
Concentration:

12.5, 25, 50 μM
Incubation Time:

24 h
Result:

Caused concentration-dependent increases in sub-G₁ hypodiploid cells.
Showed no mitotic block or delay in G₂/M phase.

Western Blot Analysis^[1]

Cell Line:

human promyelocytic leukemia HL-60 cells
Concentration:

12.5, 25 μM
Incubation Time:

24 h
Result:

Caused a slight increase in pro-apoptotic Bax protein levels, a marked decrease in anti-apoptotic Bcl-xL protein levels, an increase in active cleaved caspase-3 levels, a decrease in pro-caspase-9 levels, and cleavage of PARP, with these effects most pronounced at 25 μM.

ELISA Assay^[2]

Cell Line:

RAW 264.7 murine macrophages
Concentration:

5, 12.5, 25 μg/mL
Incubation Time:

2 h pretreatment
Result:

Dose-dependently reduced LPS-stimulated PGE₂ production.
Significantly decreased PGE₂ levels at 5 μg/mL.
Caused a greater reduction of PGE₂ production at 12.5 μg/mL.
Reduced PGE₂ production to ~20% of LPS-only treated cells at 25 μg/mL.
Dose-dependently reduced LPS-stimulated TNF-α and IL-1β production.
Significantly reduced TNF-α production at 5 μg/mL, with further decreases to ~50% of LPS-only levels at 12.5 μg/mL and 25 μg/mL.
Significantly reduced IL-1β production at 5 μg/mL and 12.5 μg/mL, and nearly completely inhibited it at 25 μg/mL.

Western Blot Analysis^[2]

Cell Line:

RAW 264.7 murine macrophages
Concentration:

5, 12.5, 25 μg/mL
Incubation Time:

2 h pretreatment
Result:

Dose-dependently suppressed LPS-stimulated COX-2 and iNOS protein expression.
Nearly completely inhibited COX-2 expression at 12.5 μg/mL and 25 μg/mL.
Significantly reduced iNOS expression at 5 μg/mL, with further dose-dependent decreases at 12.5 μg/mL and 25 μg/mL.

Western Blot Analysis^[2]

Cell Line:

RAW 264.7 murine macrophages
Concentration:

5, 12.5, 25 μg/mL
Incubation Time:

2 h pretreatment
Result:

Dose-dependently suppressed LPS-stimulated phosphorylation of ERK1/2 and JNK, and slightly reduced phosphorylation of p38.
Significantly reduced ERK1/2 phosphorylation at 5 μg/mL, with further decreases at 12.5 μg/mL and 25 μg/mL.
Strongly inhibited JNK phosphorylation at all tested concentrations, with near-complete inhibition at 25 μg/mL.
Showed a moderate, dose-dependent reduction of p38 phosphorylation.

Chemical Information

CAS No. 856414-54-5
Molecular Weight 428.60
Formula C₂₇H₄₀O₄
SMILES

C/C(C)=C\[C@@H]([C@H](/C(C)=C/CC/C(C)=C/CC[C@@]1(CCC2=CC(O)=CC(C)=C2O1)C)O)O
Structure Classification
- Lipids
Initial Source
- Marine natural products
- Marine algae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Heo SJ, et al. Chromene induces apoptosis via caspase-3 activation in human leukemia HL-60 cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2011 Sep;49(9):1998-2004.

[2]. Lee JH, et al. Preparative isolation of sargachromanol E from Sargassum siliquastrum by centrifugal partition chromatography and its anti-inflammatory activity. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2013 Dec;62:54-60.

[3]. Natarajan PM, et al. Computational simulations of identified marine-derived natural bioactive compounds as potential inhibitors of oral cancer. Future science OA. 2022 Mar;8(3):FSO782.

[4]. Birringer M, et al. Natural 6-hydroxy-chromanols and -chromenols: structural diversity, biosynthetic pathways and health implications. RSC advances. 2018 Jan 24;8(9):4803-4841.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Sargachromanol E

All Caspase Isoforms

All PARP Isoforms

All Bcl-2 Family Isoforms

All COX Isoforms

All NO Synthase Isoforms

All TNF Receptor Isoforms

All Interleukin Related Isoforms

All ERK Isoforms

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

Sargachromanol E Related Classifications

HY-123364 Related Classifications

Bioz