Cat. No.: HY-N4238

Dehydrocorydaline nitrate

Name: Dehydrocorydaline nitrate
Brand: MedChemExpress
SKU: HY-N4238
Price: 280.00 USD
Availability: InStock
Rating: 5 (45 reviews)

(Synonyms: 13-Methylpalmatine nitrate)

45 Cited Publications

Customer Review

Based on 45 publication(s) in Google Scholar

Dehydrocorydaline (13-Methylpalmatine) nitrate is an orally active alkaloid that inhibits acetylcholinesterase (AChE) (IC₅₀ = 0.62 μM). Dehydrocorydaline nitrate inhibits synaptosomal reuptake of serotonin/dopamine/norepinephrine, the NLRP3 inflammasome, microglial activation, biofilm formation, and Plasmodium/Trypanosoma growth, and has pain-relieving effects. Dehydrocorydaline nitrate activates p38 MAPK/MyoD-driven myoblast differentiation, binds SPTA1 (K_d = 2.18 µM), inhibits aldose reductase, hOCT2/3, hPMAT, MEK/ERK, NF-κB/TRAF6, IRE1/PERK, SMAD2/3, and NR2B phosphorylation, and modulates Bcl-2/Bax, caspase-8/7, and PARP signaling. Dehydrocorydaline nitrate can be used in research on pain, breast cancer, melanoma, non-small cell lung cancer, bacterial infections, parasitic infections, pulmonary fibrosis, and atherosclerosis.

For research use only. We do not sell to patients.

Purity : 99.89%
CAS No.: 13005-09-9
Formula: C₂₂H₂₄N₂O₇
Molecular Weight:428.44

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Dehydrocorydaline nitrate Chemical Structure

CAS No.: 13005-09-9

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (45)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Plasmodium

All Cholinesterase (ChE) Isoforms :

AChE
BChE

All Parasite Isoforms :

All Bcl-2 Family Isoforms :

All Caspase Isoforms :

All PARP Isoforms :

All p38 MAPK Isoforms :

All NOD-like Receptor (NLR) Isoforms :

All 5-HT Receptor Isoforms :

All Dopamine Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HuH-28	CC₅₀	100 μM	Cytotoxicity against human HuH28 cells incubated 72 hrs followed by 4 hrs alamarBlue viability assay; CC50 >100 μM. Cytotoxicity against human HuH28 cells incubated 72 hrs followed by 4 hrs alamarBlue viability assay; CC50 >100 μM.	36859380
A-375	IC50	39.73 μM	Cytotoxicity against human melanoma A375 cells measured as IC50 for viability reduction after 48 hrs by MTT assay. Cytotoxicity against human melanoma A375 cells measured as IC50 for viability reduction after 48 hrs by MTT assay.	31456643

In Vitro

Anti-cancerApoptosisCell Cycle RegulationMitochondrial FunctionBacterial InfectionCarbohydrate MetabolismProtein/Amino Acid MetabolismOxidative StressCytokines & ChemokinesEndoplasmic Reticulum StressGlutamate Release or ReceptorsInnate ImmunityInflammasomeLocal Vascular RegulationParasitic InfectionOthers

Anti-cancer

Dehydrocorydaline (20-160 μM; 48 h; 40-80 μM, 7 days) nitrate inhibits A375 and MV3 proliferation with IC₅₀ values of 39.73 and 42.34 μM, whereas PIG1 is much less sensitive (IC₅₀ 262.6 μM)^[14].
Dehydrocorydaline (40 μM; 48 h) nitrate reduces BrdU-positive A375 and MV3 melanoma cells^[14].
Dehydrocorydaline (40 μM; 0-72 h) nitrate inhibits wound closure in A375 and MV3 melanoma cells^[14].
Dehydrocorydaline (40 μM) nitrate reduces A375 and MV3 migration and Matrigel invasion^[14].
Dehydrocorydaline (40 μM; 2-3 weeks) nitrate reduces colony formation in A375 and MV3 soft agar cultures^[14].
Dehydrocorydaline (40-80 µM; 24 h) nitrate reduces H1299 motility in the wound healing assay^[15].
Dehydrocorydaline (40-80 µM; 16 h) nitrate inhibits H1299 transwell migration^[15].
Dehydrocorydaline (40-80 µM) nitrate suppresses MMP7 and MMP9 mRNA in H1299 cells^[15].
Dehydrocorydaline (40-120 µM) nitrate reduces MMP9 and MMP7 protein expression in H1299 cells^[15].
Dehydrocorydaline ((40-80 µM; 24 h) nitrate shows an anti-migratory effect that is blocked by Bcl-2 overexpression in H1299 cells^[15].

Anti-cancer

Apoptosis

Dehydrocorydaline (0-200 μM; 24 h) nitrate inhibits MCF-7 proliferation, producing approximately 40% viability loss at 200 μM after 24 h^[4].
Dehydrocorydaline (200 μM; 24-72 h) nitrate induces time-dependent apoptotic nuclear morphology in MCF-7 cells^[4].
Dehydrocorydaline (0-200 μM; 72 h) nitrate increases DNA fragmentation in MCF-7 cells in a time-dependent manner, especially at 72 h^[4].
Dehydrocorydaline (0-200 μM) nitrate regulates Bax/Bcl-2 and activates caspase-7, caspase-8, and PARP without activating caspase-9 in MCF-7 cells^[4].
Dehydrocorydaline (40-120 µM) nitrate decreases Bcl-2 and increases Bax protein in H1299, H596, H460, and A549 NSCLC cells^[15].

Anti-cancer

Cell Cycle Regulation

Dehydrocorydaline (20-80 μM; 48 h) nitrate arrests A375 and MV3 cells at G0/G1, while 40 μM does not significantly arrest PIG1 cells^[14].
Dehydrocorydaline (20-80 μM; 48 h; 24-72 h) nitrate decreases CDK6 and cyclin D1 protein levels in A375 and MV3 melanoma cells in dose- and time-dependent manners^[14].
Dehydrocorydaline (20-80 μM; 48 h; 24-72 h) nitrate increases E-cadherin and decreases vimentin and β-catenin in A375 and MV3 melanoma cells^[14].
Dehydrocorydaline (20-80 μM; 48 h; 24-72 h) nitrate decreases phospho-MEK1/2 and phospho-ERK1/2 levels in A375 and MV3 melanoma cells^[14].

Anti-cancer

Mitochondrial Function

Dehydrocorydaline (0-200 μM; 1 h) nitrate does not alter mitochondrial membrane potential in MCF-7 cells^[4].

Apoptosis

Dehydrocorydaline (5-20 μg/mL) nitrate dose-dependently shifts apoptosis-related protein expression toward reduced apoptosis after LPS exposure in H9C2 cardiomyocytes^[10].
Dehydrocorydaline (5-20 μg/mL) nitrate reverses LPS-induced iNOS upregulation and Nrf2/HO-1 suppression in H9C2 cardiomyocytes^[10].
Dehydrocorydaline (5-20 μg/mL) nitrate dose-dependently attenuates LPS-induced TRAF6/NF-κB activation in H9C2 cardiomyocytes^[10].

Bacterial Infection

Dehydrocorydaline (nitrate) (0.01-2 mg/mL; 24 h) nitrate inhibits L. monocytogenes ATCC 7644 with MIC 1 mg/mL and MBC 2 mg/mL and shows activity against the other tested bacterial strains, with the lowest MIC against S. aureus ATCC 25923 at 0.5 mg/mL^[8].
Dehydrocorydaline (nitrate) (0-4 mg/mL; 24 h) nitrate inhibits L. monocytogenes ATCC 7644 planktonic growth in a concentration-dependent manner, with MIC 1 mg/mL and MBC 2 mg/mL suppressing growth over 24 h^[8].
Dehydrocorydaline (0-2 mg/mL; 0-4 h) nitrate disrupts L. monocytogenes ATCC 7644 cytoplasmic membrane integrity, causing nucleic acid and protein leakage at MIC 1 mg/mL and MBC 2 mg/mL^[8].
Dehydrocorydaline (0-2 mg/mL; 4 h) nitrate at MIC 1 mg/mL and MBC 2 mg/mL causes membrane shrinkage and cell-wall/membrane destruction in L. monocytogenes ATCC 7644^[8].
Dehydrocorydaline (0-1 mg/mL; 24 h) nitrate reduces L. monocytogenes ATCC 7644 biofilm formation at 0.25, 0.5, and 1 mg/mL over 24 h^[8].
Dehydrocorydaline (0-1 mg/mL) nitrate reduces metabolic activity in L. monocytogenes ATCC 7644 biofilms at 0.25, 0.5, and 1 mg/mL^[8].
Dehydrocorydaline (0-1 mg/mL; 24 h) nitrate inhibits L. monocytogenes ATCC 7644 swimming motility at 0.25, 0.5, and 1 mg/mL and swarming motility at 0.5 and 1 mg/mL^[8].

Bacterial Infection

Carbohydrate Metabolism

Dehydrocorydaline (0.75 mg/mL) nitrate alters mRNA levels of ManZ, ManXa, GPI, ScrK, Pyc, GltA, GlmS, MurG, FliE, and FliG in L. monocytogenes, as confirmed by qRT-PCR in concordance with LFQ^[8].

Bacterial Infection

Carbohydrate Metabolism

Protein/Amino Acid Metabolism

Dehydrocorydaline(0.25 mg/mL; 2 h) nitrate induces 444 DEPs in L. monocytogenes, including downregulation of PTS, cell-wall synthesis, and flagellar proteins, supporting multi-target antibacterial action^[8].

Bacterial Infection

Oxidative Stress

Dehydrocorydaline (0.25-2 mg/mL; 1 h) nitrate increases intracellular ROS in L. monocytogenes ATCC 7644 in a dose-dependent manner, with significant fluorescence at 0.5, 1, and 2 mg/mL (1/2 MIC, MIC, and MBC)^[8].

Cytokines & Chemokines

Dehydrocorydaline (10-30 μM) nitrate suppresses TGF-β1-induced fibrotic gene expression in HPFs^[12].
Dehydrocorydaline (10-30 μM) nitrate reduces TGF-β1-induced Fibronectin, Collagen 1, and α-SMA protein in HPFs^[12].
Dehydrocorydaline (10-30 μM) nitrate attenuates TGF-β1-induced fibrotic marker immunofluorescence in HPFs^[12].
Dehydrocorydaline (2 h) nitrate suppresses TGF-β1-induced SMAD2/3 phosphorylation in HPFs without affecting PI3K-AKT-mTOR or MAPK/ERK^[12].

Cytokines & Chemokines

Endoplasmic Reticulum Stress

Dehydrocorydaline (10 μM; 24 h) nitrate suppresses TGF-β1-induced Fibronectin, Collagen 1, and IRE1/PERK ER-stress markers in HPFs^[12].

Cytokines & Chemokines

Oxidative Stress

Dehydrocorydaline (5-20 μg/mL) nitrate dose-dependently suppresses LPS-induced inflammatory/oxidative injury in H9C2 cardiomyocytes^[10].
Dehydrocorydaline (10 μM; 24 h) reduces TGF-β1-induced ROS generation in HPFs^[12].

Glutamate Release or Receptors

Dehydrocorydaline (500 nM for 4 h) nitrate suppresses LPS-induced p-NR2B/NR2B upregulation in mouse primary spinal neurons, consistent with reduced NMDA receptor NR2B phosphorylation^[16].

Innate Immunity

Dehydrocorydaline (0-200 μM; 24 h) nitrate is non-cytotoxic to BMDMs below 100 μM and cytotoxic at 200 μM^[9].
Dehydrocorydaline (100 μM) nitrate inhibits LPS-induced p65 and ERK1/2 phosphorylation without affecting p38 or JNK phosphorylation in BMDMs^[9].
Dehydrocorydaline nitrate inhibits LPS-induced p65 nuclear translocation in BMDMs^[9].
Dehydrocorydaline (5-20 μg/mL) nitrate selectively reduces TRAF6 expression in H9C2 cardiomyocytes^[10].

Innate Immunity

Cytokines & Chemokines

Dehydrocorydaline (100 μM) nitrate downregulates inflammatory gene clusters in BMDMs^[9].
Dehydrocorydaline (50-100 μM; 6-12 h) nitrate decreases IL-1β and IL-18 mRNA in resting BMDMs in a time- and concentration-dependent manner^[9].
Dehydrocorydaline (50-100 μM; 12-24 h) nitrate decreases CD80 and IL-18 protein levels in unstimulated BMDMs^[9].
Dehydrocorydaline nitrate reduces inflammatory factor mRNA and VCAM1 protein in rat VSMCs^[11].
Dehydrocorydaline (50 μM; 48 h) nitrate attenuates LPS-induced p65 and TNF-α protein expression in SV40-transfected human chondrocytes, consistent with inhibition of NF-κB pro-inflammatory activation, while IL-6 could not be reliably detected^[13].

Innate Immunity

Cytokines & Chemokines

Inflammasome

Oxidative Stress

Dehydrocorydaline nitrate suppresses LPS/IFNγ-induced CD80, iNOS, NLRP3, IL-1β, and IL-18 protein expression in BMDMs^[9].

Innate Immunity

Cytokines & Chemokines

Oxidative Stress

Apoptosis

Dehydrocorydaline (10 μg/mL) nitrate does not add to BAY 11-7082-mediated protection in LPS-induced H9C2 cardiomyocytes, consistent with NF-κB dependence downstream of TRAF6^[10].

Local Vascular Regulation

Dehydrocorydaline (100 μM; 24 h) nitrate upregulates 47 genes and downregulates 51 genes, enriching smooth muscle contraction pathways and increasing Cnn1, Sm22α, and Acta2 expression in rat VSMCs^[11].
Dehydrocorydaline (50-100 μM; 12-24 h) nitrate increases Cnn1, Myh11, Sm22α, and Acta2 mRNA in a time- and concentration-dependent manner in rat VSMCs^[11].
Dehydrocorydaline (50-100 μM; 12-24 h) nitrate upregulates Cnn1 and SM-MHC protein in rat VSMCs^[11].
Dehydrocorydaline (100 μM; 24 h) nitrate increases α-SMA immunofluorescence in rat VSMCs^[11].
Dehydrocorydaline (100 μM) nitrate upregulates contractile markers in rat VSMCs; Spta1 knockdown prevents this upregulation, indicating that Spta1 mediates the effect^[11].

Local Vascular Regulation

Cytokines & Chemokines

Dehydrocorydaline (nitrate) (100 μM; 15 min pre-exposure; 24 h co-exposure) prevents PDGF-BB-induced loss of α-SMA immunofluorescence in rat VSMCs^[11].
Dehydrocorydaline (100 μM; 36 h) nitrate inhibits PDGF-BB-induced migration in a wound-healing assay in rat VSMCs^[11].

Parasitic Infection

Dehydrocorydaline (10 µM; 96 h) nitrate inhibits P. falciparum 3D7 blood-stage growth with an IC₅₀ of 38 nM (36-39) and 99.7% inhibition at 10 µM^[1].
Dehydrocorydaline (96 h) nitrate inhibits the multidrug-resistant P. falciparum Dd2 strain with an IC₅₀ of 161 nM and 94.5% growth inhibition^[1].
Dehydrocorydaline (0.001-10 µM; 96 h) nitrate shows low cytotoxicity to HEK 293T cells (>90% viability up to 10 µM) with a parasite selectivity of >14,900^[1].
Dehydrocorydaline (0-48 h) nitrate inhibits P. falciparum 3D7 development at the trophozoite and schizont stages^[1].
Dehydrocorydaline (72 h) nitrate inhibits luciferase-expressing T. cruzi trypomastigotes/amastigotes in NMH coculture with an IC₅₀ of 3.47 μM^[2].
Dehydrocorydaline (72 h) nitrate inhibits intracellular T. cruzi amastigotes in NMH cells with an IC₅₀ of 7.89 μM^[2].
Dehydrocorydaline (72 h) nitrate inhibits T. cruzi epimastigotes with an IC₅₀ of 17.3 μM^[2].

Others

Dehydrocorydaline (72 h) nitrate has low cytotoxicity against NMH and HuH28 cells with a CC₅₀ >40 μM^[2].
Dehydrocorydaline (10 nM; 24-48 h) nitrate is the active phytochemical from Corydalis tuber in the C2C12 MyoD reporter and MHC induction screen^[3].
Dehydrocorydaline (125-500 nM; 2-3 days) nitrate increases MHC, MyoD, and myogenin expression in C2C12 myoblasts without markedly changing Cdo^[3].
Dehydrocorydaline (125-500 nM; 3 days) nitrate promotes multinucleated myotube formation in C2C12 myoblasts^[3].
Dehydrocorydaline (125-500 nM; 2-3 days) nitrate activates p38 MAPK in differentiating C2C12 myoblasts, with elevated p-p38 at day 2 and day 3^[3].
Dehydrocorydaline (500 nM; 1-3 days) nitrate increases p-p38 immunofluorescence 2.34-fold in differentiating C2C12 myoblasts^[3].
Dehydrocorydaline (500 nM; differentiation day 2) nitrate enhances MyoD-E2A interaction in differentiating C2C12 myoblasts^[3].
Dehydrocorydaline (500 nM; 24 h) nitrate increases MyoD-E2A binding in MyoD-transfected 10T1/2 fibroblasts^[3].
Dehydrocorydaline (500 nM; 24 h) nitrate increases MyoD-E2A binding in MyoD-transfected 293T cells^[3].
Dehydrocorydaline (500 nM; 2 days) nitrate rescues Cdo depletion-induced reductions in muscle-specific protein expression and p38 MAPK activation in C2C12 myoblasts^[3].
Dehydrocorydaline (500 nM; 3 days) nitrate improves defective myotube formation in Cdo-depleted C2C12 myoblasts^[3].
Dehydrocorydaline (0.0001-100 μM; 3-30 min) nitrate selectively inhibits uptake-2 transporters hOCT2, hOCT3, and hPMAT in transfected MDCK cells with IC₅₀ values of 0.137-3.70 μM, without inhibiting hSERT or hDAT at 100 μM^[5].
Dehydrocorydaline (12.5-50 μM; 10 min) nitrate inhibits synaptosomal 5-HT, DA, and NE reuptake in mouse brain synaptosomes, producing approximately 52.2%, 39.9%, and 38.3% inhibition at 50 μM^[5].
Dehydrocorydaline (5-20 μg/mL) nitrate dose-dependently restores LPS-reduced viability and proliferation in H9C2 cardiomyocytes^[10].
Dehydrocorydaline nitrate binds SPTA1 with a K_d of 2.18 μM in an SPR assay^[11].
Dehydrocorydaline (100 μM; 24 h) nitrate upregulates Spta1/SPTA1 expression, with Spta1 identified among the top induced differentially expressed genes by RNA-seq in rat VSMCs^[11].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

C2C12 mouse myoblasts
Concentration:

125, 250, and 500 nM (2-day differentiation); 500 nM (differentiation day 2 and day 3)
Incubation Time:

2 days (differentiation); day 2 and day 3 (500 nM)
Result:

Increased MHC, MyoD, and myogenin expression in a dose-dependent manner compared with DMSO control cells; Cdo levels were not markedly altered.
At 500 nM, increased MHC, MyoD, and myogenin at day 2 and day 3.

Immunofluorescence^[3]

Cell Line:

C2C12 mouse myoblasts
Concentration:

125, 250, and 500 nM
Incubation Time:

3 days (differentiation)
Result:

Formed larger myotubes with more nuclei per myotube than control cells, with an increase in myotubes containing ≥10 nuclei relative to vehicle control cells.

Western Blot Analysis^[3]

Cell Line:

C2C12 mouse myoblasts
Concentration:

125, 250, and 500 nM (differentiation); 500 nM (differentiation day 2 and day 3)
Incubation Time:

during differentiation; day 2 and day 3 (500 nM)
Result:

Upregulated p-p38 in a dose-dependent manner.
At 500 nM, elevated p-p38 was observed at differentiation day 2 and day 3 compared with vehicle control cells.

Immunofluorescence^[3]

Cell Line:

C2C12 mouse myoblasts
Concentration:

500 nM
Incubation Time:

1 day (Results); 2 or 3 days (Methods)
Result:

Increased p-p38-positive cells compared with control cells.
p-p38 signal intensity was 2.34-fold higher relative to DMSO control cells.

Western Blot Analysis^[3]

Cell Line:

C2C12 mouse myoblasts (Cdo-depleted and control)
Concentration:

500 nM
Incubation Time:

2 days (differentiation)
Result:

Cdo-depleted cells had decreased Cdo, MHC, MyoD, and p-p38 compared with C2C12/pSuper control cells.
Restored MHC, MyoD, and myogenin expression in C2C12/Cdo shRNA cells to levels similar to those in C2C12/pSuper cells.
C2C12/Cdo shRNA cells exhibited slightly higher p-p38 levels than C2C12/pSuper cells, whereas total p38 remained constant.

Immunofluorescence^[3]

Cell Line:

C2C12 mouse myoblasts (Cdo-depleted and control)
Concentration:

500 nM
Incubation Time:

3 days (differentiation)
Result:

C2C12/pSuper cells exhibited enhanced myotube formation, including myotubes containing ≥10 nuclei, compared with control C2C12/pSuper cells.
Vehicle control C2C12/Cdo shRNA cells exhibited reduced myotube formation, which was markedly improved almost to control cell levels.

ELISA Assay^[10]

Cell Line:

H9C2 rat embryonic ventricular cardiomyocytes
Concentration:

20 μg/mL (alone); 5, 10, 20 μg/mL (with LPS 10 μg/mL); 20 µM (DCFDA)
Incubation Time:

45 min (DCFDA)
Result:

At 5, 10, and 20 μg/mL with LPS, reduced IL-1β and TNFα and increased SOD and GSH-PX dose-dependently compared with LPS.
At 5, 10, and 20 μg/mL with LPS, reduced ROS dose-dependently compared with LPS.

Western Blot Analysis^[13]

Cell Line:

SV40-transfected human immortalized chondrocytes (CTCC-001-0662)
Concentration:

50 μM
Incubation Time:

48 h (after 24 h LPS)
Result:

Relative p65 protein expression was increased in the LPS group versus Control and significantly reduced relative to LPS at 50 μM.
Relative TNF-α protein expression was increased in the LPS group versus Control and significantly reduced relative to LPS at 50 μM.
IL-6 data required further validation because no clear detectable band was observed.

Cell Viability Assay^[14]

Cell Line:

human melanoma A375 and MV3 cells; normal melanocyte PIG1 cells
Concentration:

20, 40, 80 μM (A375/MV3); 20, 40, 80, 160 μM (PIG1); six doses (IC50); 40 and 80 μM (growth curves)
Incubation Time:

48 h (A375/MV3, PIG1, IC50); 7 days (growth curves)
Result:

IC50 values were 39.73 μM in A375, 42.34 μM in MV3, and 262.6 μM in PIG1.
Proliferation was inhibited in A375 and MV3 in a dose-dependent manner at 20, 40, and 80 μM after 48 h.
Growth was markedly suppressed in A375 and MV3 at 40 and 80 μM over 7 days.
No significant proliferation inhibition was observed in PIG1 after 20 or 40 μM for 48 h.

Cell Proliferation Assay^[14]

Cell Line:

human melanoma A375 and MV3 cells
Concentration:

40 μM; BrdU 10 μg/mL
Incubation Time:

48 h; 0.5 h (BrdU)
Result:

The ratio of BrdU-positive A375 and MV3 cells was significantly decreased compared with control groups at 40 μM after 48 h.

Cell Cycle Analysis^[14]

Cell Line:

human melanoma A375 and MV3 cells; normal melanocyte PIG1 cells
Concentration:

20, 40, 80 μM (A375/MV3); 40 μM (PIG1)
Incubation Time:

48 h
Result:

Significant G0/G1 phase arrest was induced in A375 and MV3 cells in a dose-dependent manner at 20, 40, and 80 μM after 48 h.
No significant cell cycle arrest was induced in PIG1 cells at 40 μM after 48 h.

Western Blot Analysis^[14]

Cell Line:

human melanoma A375 and MV3 cells
Concentration:

20, 40, 80 μM (dose response)
Incubation Time:

48 h (dose response); 24, 48, 72 h (time course)
Result:

CDK6 and cyclin D1 protein levels were decreased in both dose- and time-dependent manners in A375 and MV3 cells.\nE-cadherin expression was upregulated and vimentin and β-catenin expression was downregulated in A375 and MV3 cells in both dose- and time-dependent manners.\nPhosphorylation of MEK1/2 at Ser221 and ERK1/2 at Thr202/Tyr204 was downregulated in A375 and MV3 cells in both dose- and time-dependent manners.

Cell Migration Assay^[14]

Cell Line:

human melanoma A375 and MV3 cells
Concentration:

40 μM
Incubation Time:

0, 24, 48, 72 h
Result:

The rate of wound closure was significantly blocked in A375 and MV3 cells compared with control groups at 40 μM.

Cell Proliferation Assay^[14]

Cell Line:

human melanoma A375 and MV3 cells
Concentration:

40 μM
Incubation Time:

2 weeks (MV3); 3 weeks (A375)
Result:

A375 and MV3 cells formed smaller and less numerous colonies than control groups at 40 μM.

Cell Proliferation Assay^[14]

Cell Line:

human melanoma A375 and MV3 cells
Concentration:

50 μM (tBHQ)
Incubation Time:

0, 2, 4, 6 days
Result:

tBHQ at 50 μM recovered inhibition of A375 and MV3 cell proliferation.

Cell Cytotoxicity Assay^[15]

Cell Line:

H1299, H596, A549, and H460 NSCLC cells
Concentration:

0, 10, 20, 40, 80, 120, 160 µM
Incubation Time:

24 h
Result:

Weak cytotoxicity occurred in H1299, H596, A549, and H460 NSCLC cells at 0, 10, 20, 40, 80, 120, and 160 µM after 24 h.

Cell Migration Assay^[15]

Cell Line:

H1299 NSCLC cells
Concentration:

40, 80 µM
Incubation Time:

24 h
Result:

At 80 µM, H1299 cell motility was significantly reduced compared with untreated control.

Cell Migration Assay^[15]

Cell Line:

H1299 NSCLC cells
Concentration:

40, 80 µM
Incubation Time:

16 h
Result:

At 40 and 80 µM, an anti-migratory effect reduced migrated cell numbers in H1299 cells compared with untreated control.

Cell Migration Assay^[15]

Cell Line:

pcDNA- and pcDNA-Bcl-2-transfected H1299 NSCLC cells
Concentration:

40, 80 µM
Incubation Time:

24 h
Result:

At 40 and 80 µM, Bcl-2 overexpression blocked the anti-migratory effect in pcDNA-Bcl-2-transfected H1299 cells compared with pcDNA control.

In Vivo

Anti-cancerCell Cycle RegulationInnate ImmunityCytokines & ChemokinesOxidative StressBacterial InfectionApoptosisRenal RegulationInflammasomeLipid MetabolismLocal Vascular RegulationGlutamate Release or ReceptorsOthers

Anti-cancer

Cell Cycle Regulation

Dehydrocorydaline (100 mg/kg; p.o.; every 24 h; 12 days) nitrate suppresses melanoma xenograft growth and reduces tumor weight in BALB/c-nu mice^[14].

Anti-cancer

Innate Immunity

Cytokines & Chemokines

Oxidative Stress

Dehydrocorydaline (10 mg/kg; i.p.; single administration; day 14 after osteosarcoma cell implantation) nitrate attenuates pain behavior and shifts spinal microglial polarization toward the M2 phenotype with reduced pro-inflammatory and increased anti-inflammatory markers in a mouse bone cancer pain model^[7].

Bacterial Infection

Apoptosis

Cytokines & Chemokines

Oxidative Stress

Renal Regulation

Innate Immunity

Dehydrocorydaline (5-20 mg/kg; intraperitoneal; once every 24 h; three consecutive days) nitrate attenuates sepsis-induced myocardial injury in C57B6/L mice, improving survival and reducing cardiac apoptosis, inflammation, oxidative stress, and TRAF6/NF-κB activation^[10].

Cytokines & Chemokines

Dehydrocorydaline (3.6-10 mg/kg; i.p.; single dose; 15 min before formalin) nitrate dose-dependently reduces formalin-induced nociception and paw edema and lowers spinal CASP6/TNF-α/IL-1β/IL-6 in mice^[6].

Innate Immunity

Cytokines & Chemokines

Inflammasome

Lipid Metabolism

Local Vascular Regulation

Dehydrocorydaline (5 mg/kg; i.p.; daily; 12 weeks; i.p.; daily; 1 week) nitrate ameliorates atherosclerosis in Western diet-fed ApoE^−/− mice, improving aortic compliance and plaque stability while reducing systemic and vascular inflammation^[9].

Innate Immunity

Cytokines & Chemokines

Oxidative Stress

Glutamate Release or Receptors

Dehydrocorydaline (10 mg/kg; intrathecal injection; single dose; day 14 after CCI) nitrate relieves CCI-induced neuropathic pain in mice by attenuating spinal neuroinflammation and neuronal hyperactivity^[16].

Innate Immunity

Local Vascular Regulation

Dehydrocorydaline (5 mg/kg; i.p.; daily) nitrate attenuates carotid atherosclerosis and maintains the VSMC contractile phenotype in Apoe^−/− mice^[11].

Others

Dehydrocorydaline (1.5-3 mg/kg; intraperitoneal injection; once daily; 21 days) nitrate produces behavioral effects in male ICR mice with CUMS and increases prefrontal cortex 5-HT and NE levels while decreasing their turnover ratios^[5].
Dehydrocorydaline (3.6-10 mg/kg; i.p.; single dose; 15 min before acetic acid) nitrate dose-dependently reduces acetic acid-induced writhing in mice^[6].
Dehydrocorydaline (10 mg/kg; i.p.; single dose; 15 min before formalin) nitrate antinociception in the formalin test is opioid receptor-dependent, being completely blocked by naloxone in Phase 1 and partially blocked in Phase 2 in mice^[6].
Dehydrocorydaline (5-10 mg/kg; i.p.; every other day; days 12, 14, 16, 18, and 20) nitrate attenuates bleomycin-induced pulmonary fibrosis in C57BL/6 mice in a dose-dependent manner, reducing fibrosis scores, hydroxyproline content, and fibrotic marker expression^[12].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

ICR (male, 6 weeks old, 18-20 g)^[5]
Dosage:

1.5 mg/kg; 3 mg/kg
Administration:

intraperitoneal injection; once daily; 21 days
Result:

In the sucrose preference test, 3 mg/kg increased sucrose preference relative to the CUMS control group.
In the open-field test, 1.5 and 3 mg/kg increased time in the central region, frequency of passing across the center, and frequency of upright behavior relative to the CUMS control group to different extents.
In the forced swimming test, 1.5 and 3 mg/kg decreased immobility duration relative to the CUMS control group.
In prefrontal cortex biochemistry, 3 mg/kg increased 5-HT levels and decreased the 5-HIAA/5-HT ratio relative to the CUMS control group.
1.5 and 3 mg/kg increased NE levels and decreased the MHPG/NE ratio.
3 mg/kg decreased DA levels and increased the DOPAC/DA ratio relative to the CUMS control group.

Animal Model:

ICR (adult male, 25-35 g)^[6]
Dosage:

3.6 mg/kg; 6 mg/kg; 10 mg/kg
Administration:

i.p.; single dose; 15 min before acetic acid
Result:

Delayed the onset time of writhing dose-dependently.
Reduced acetic acid-induced writhing episodes by 16.5%, 34.0%, and 52.6% at 3.6, 6, and 10 mg/kg, respectively, compared with control.

Animal Model:

ICR (adult male, 25-35 g)^[6]
Dosage:

3.6 mg/kg; 6 mg/kg; 10 mg/kg
Administration:

i.p.; single dose; 15 min before formalin
Result:

Produced a dose-dependent reduction in Phase 2 licking/biting time; only the 10 mg/kg dose showed an antinociceptive effect in Phase 1.
At 10 mg/kg, reduced formalin-induced paw edema by 66.8%, with paw thickness of 0.67 mm.
Dose-dependently down-regulated spinal p-CASP6, a-CASP6, TNF-α, IL-1β, and IL-6; at 10 mg/kg, p-CASP6 was decreased by 59%, a-CASP6 by 67%, TNF-α by 77%, IL-1β by 65%, and IL-6 by 81%.

Animal Model:

ICR (adult male, 25-35 g)^[6]
Dosage:

10 mg/kg; 2 mg/kg (naloxone)
Administration:

i.p.; single dose; 15 min before formalin; naloxone i.p. before Dehydrocorydaline (nitrate)
Result:

10 mg/kg alone produced antinociceptive activity in both Phase 1 and Phase 2.
Naloxone completely prevented the Phase 1 antinociceptive effect and partially prevented the Phase 2 effect.

Animal Model:

C3H/HeN (male, 4-6 weeks old, 20-25 g)^[7]
Dosage:

10 mg/kg
Administration:

i.p.; single administration; day 14 after osteosarcoma cell implantation
Result:

At 1 h after administration, PWMT was higher and NSF was lower than in vehicle-treated mice.
At 4 h, PWMT remained higher and NSF remained lower than in vehicle-treated mice.
By 8 h both returned to preinjection levels.
Spinal iNOS was reduced at 1 h and 4 h.
Spinal Arg-1 was increased at 1 h.
CD16/32 was reduced at 1 h and 4 h with recovery by 8 h.
CD206 was increased at 1 h with return to preinjection levels by 4 h.
Iba-1 did not substantially change.
Arg-1 immunofluorescence changed similarly to CD206.
Spinal IL-1β was reduced at 1 h and 4 h.
Spinal IL-10 was increased at 1 h and 4 h, with recovery by 8 h.
ELISA showed corresponding IL-1β and IL-10 changes in spinal cord and blood.

Animal Model:

ApoE−/− (male, 8 weeks old)^[9]
Dosage:

5 mg/kg
Administration:

i.p.; daily; 12 weeks; i.p.; daily; 1 week (short-term aortic inflammation assessment)
Result:

Peak diastolic velocity was decreased in the ascending aorta, aortic arch, and brachiocephalic artery.
B-mode echocardiography showed reduced atherosclerotic plaque formation in the aorta.
Aortic root lesion area, oil red O+ area, and F4/80+ macrophage area were reduced.
Collagen content was increased.
α-SMA+ area was not altered.
The plaque vulnerability index was decreased.
In the 5 mg/kg group, plasma TG was 1.3 mmol/L, TC was 28.4 mmol/L, LDL-C was 11.3 mmol/L, HDL-C was 1.5 mmol/L, and body weight was 29.7 g (n = 7 mice per group).
TG decreased and LDL-C increased, while HDL-C, TC, and body weight were comparable.
Plasma TNFα and IL-1β levels were reduced.
Short-term i.p. administration for 1 week reduced aortic TNFα, NLRP3, and IL-18 mRNA levels.

Animal Model:

C57B6/L (male, 8-10 weeks old, 20-22 g)^[10]
Dosage:

5 mg/kg, 10 mg/kg, 20 mg/kg
Administration:

intraperitoneal; once every 24 h; for three consecutive days; starting 30 min after E. coli injection
Result:

Survival rate increased, with significance when the dose was over 10 mg/kg.
Reduced bacterial counts in peritoneal lavage fluid and serum.
Reduced serum creatinine, urea nitrogen, AST, and ALT.
H&E and Masson staining showed reduced disordered myocardial structure, inflammatory cell infiltration, and collagen fibers.
TUNEL staining showed fewer TUNEL-positive cardiomyocytes.
In heart tissue, Bax and Caspase3 decreased and Bcl2 increased in a dose-dependent manner.
Reduced plasma IL-1β, TNFα, IL-6, and IFNγ and increased SOD and GSH-PX in a dose-dependent manner.
In heart tissue, iNOS decreased and cytoplasmic Nrf2, HO-1, and nuclear Nrf2 were promoted.
Reduced TRAF6 expression in heart tissue and decreased cytoplasmic and nuclear p-NF-κB.

Animal Model:

Apoe−/− (male)^[11]
Dosage:

5 mg/kg
Administration:

i.p.; daily
Result:

In Apoe−/− mice with carotid injury, carotid plaque area and F4/80 macrophage infiltration in plaques were reduced, and plaque α-SMA and SM-MHC mean fluorescence intensity was increased compared with vehicle.

Animal Model:

C57BL/6 (male; 6-8 weeks old; average weight 25 g)^[12]
Dosage:

5 mg/kg; 10 mg/kg
Administration:

i.p.; every other day on days 12, 14, 16, 18, and 20
Result:

In bleomycin-induced pulmonary fibrosis mice, lung damage and collagen deposition on H&E, Masson’s trichrome, and Sirius Red staining were reduced in a dose-dependent manner at 5 mg/kg and 10 mg/kg.
Reduced Ashcroft scores relative to BLM+DMSO.
Reduced lung hydroxyproline content relative to BLM+DMSO.
Reduced Collagen 1, Collagen 3, α-SMA, and Fibronectin expression by Western blot and RT-qPCR relative to BLM+DMSO.
Immunofluorescence showed reduced Collagen 1, Fibronectin, and α-SMA relative to BLM+DMSO.

Animal Model:

BALB/c-nu (nude) (female, 4 weeks old; n=6)^[14]
Dosage:

100 mg/kg
Administration:

p.o.; every 24 h; 12 days
Result:

Tumor volume was suppressed in A375 and MV3 melanoma xenografts compared with DMSO controls.
Excised tumor weights were lower than in DMSO control mice.
Immunohistochemistry showed reduced Ki-67, Cyclin D1, and vimentin expression in A375 and MV3 xenograft tumors.
H&E staining showed inhibited cell proliferation in xenografts.

Animal Model:

C57BL/6J (adult male, specific-pathogen-free, 20-25 g)^[16]
Dosage:

10 mg/kg
Administration:

intrathecal injection into the L5-L6 intervertebral space; single dose on day 14 after CCI
Result:

A single intrathecal injection increased PWMT in CCI mice at 3-5 h after injection compared with CCI controls; PWMT decreased by 6 h after injection.
Downregulated spinal protein expression of iNOS, TNF-α, and IL-6 compared with CCI.
Reduced Iba1-positive microglial and GFAP-positive astrocyte activation in the spinal cord compared with CCI.
Reduced the spinal p-NR2B/NR2B ratio compared with CCI.

Related Protocols :

Chemical Information

CAS No. 13005-09-9
Appearance Solid
Molecular Weight 428.44
Formula C₂₂H₂₄N₂O₇
Color Yellow to orange
SMILES

[O-][N+]([O-])=O.CC1=C(C=CC(OC)=C2OC)C2=C[N+]3=C1C4=CC(OC)=C(OC)C=C4CC3
Synonyms

13-Methylpalmatine nitrate
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
Initial Source
- Plant
- Other Families Extract
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (45)

All Histological Imaging/StainingRT-PCRIFCell Proliferation/Viability AssayWB

Sort by:

Journal Impact Factor

J Nanobiotechnology

A fibrin gel-loaded Gouqi-derived nanovesicle (GqDNV) repairs the heart after myocardial infarction by inhibiting p38 MAPK/NF-κB p65 pathway. [Abstract]

2025 Jul 22;23(1):535. PMID: 40696398
J Clin Invest

Myostatin regulates energy homeostasis through autocrine- and paracrine-mediated microenvironment communications. [Abstract]

2024 Jun 18;134(16):e178303. PMID: 38889010
Phytomedicine

The role of daurisoline treatment in hepatocellular carcinoma: Inhibiting vasculogenic mimicry formation and enhancing sensitivity to sorafenib. [Abstract]

2021 Nov:92:153740. PMID: 34600176
Mater Today Bio

Dual targeted gene delivery strategy mediated by GalNAc-modified lipid nanoparticles enhances liver regeneration through specific knockdown of MKK4. [Abstract]

2026 Mar 24:38:103059. PMID: 41970250
New Phytol

LbSakA-mediated phosphorylation of the scaffolding protein LbNoxR in the ectomycorrhizal basidiomycete Laccaria bicolor regulates NADPH oxidase activity, ROS accumulation and symbiosis development. [Abstract]

2024 Jul;243(1):381-397. PMID: 38741469
Phytother Res

Dehydrocorydaline Promotes STAT3/Bcl-2 Axis-Mediated Macrophage Efferocytosis to Attenuate Apoptosis in Atherosclerotic Plaques. [Abstract]

2026 May;40(5):2473-2490. PMID: 41699991
J Ethnopharmacol

Dehydrocorydaline attenuates myocardial ischemia-reperfusion injury via the FoXO signalling pathway: A multimodal study based on network pharmacology, molecular docking, and experimental study. [Abstract]

2025 Jan 30;337(Pt 1):118738. PMID: 39222757
J Agric Food Chem

Sulforaphene Inhibits Periodontitis through Regulating Macrophage Polarization via Upregulating Dendritic Cell Immunoreceptor. [Abstract]

2023 Oct 25;71(42):15538-15552. PMID: 37823224
Ecotoxicol Environ Saf

Dibutyl phthalate promotes central precocious puberty through primordial follicle activation by downregulating BMP15. [Abstract]

2025 Jul 15:300:118460. PMID: 40472697
Biochem Pharmacol

STM2457 decreases m6A methylation to reduce cisplatin-induced ototoxicity via MAPK signaling. [Abstract]

2025 May:235:116820. PMID: 39983847
Biochem Pharmacol

Ursodeoxycholic acid alleviates fat embolism syndrome-induced acute lung injury by inhibiting the p38 MAPK/NF-κB signalling pathway through FXR. [Abstract]

2024 Dec;230(Pt 1):116574. PMID: 39396648
Mol Metab

TRPC6 governs brown adipose thermogenesis via a BMPR2-p38 MAPK signaling axis. [Abstract]

2026 Apr:106:102341. PMID: 41747880
Respir Res

Dehydrocorydaline attenuates bleomycin-induced pulmonary fibrosis by inhibiting fibroblast activation. [Abstract]

2025 Apr 12;26(1):136. PMID: 40221718

Dehydrocorydaline nitrate purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 Apr 12;26(1):136. [Abstract]

Images of mice lung tissue slices stained with H&E, Masson, and Sirius Red treated with L-DHC (Dehydrocorydaline) 5 mg/kg, H-DHC: 10 mg/kg.

Dehydrocorydaline nitrate purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 Apr 12;26(1):136. [Abstract]

RT-qPCR results for Col1a1 and Fn1 expression levels of mice lung tissue treated with L-DHC (Dehydrocorydaline) 5 mg/kg, H-DHC: 10 mg/kg.

Dehydrocorydaline nitrate purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 Apr 12;26(1):136. [Abstract]

Collagen 1 and Fibronectin of mice lung tissues are analyzed using Western blot treated with L-DHC (Dehydrocorydaline) 5 mg/kg, H-DHC: 10 mg/kg.

Dehydrocorydaline nitrate purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 Apr 12;26(1):136. [Abstract]

Immunofluorescence images of α-SMA, Collagen 1, and Fibronectin in mice lung tissue slices treated with L-DHC (Dehydrocorydaline) 5 mg/kg, H-DHC: 10 mg/kg.

Dehydrocorydaline nitrate purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 Apr 12;26(1):136. [Abstract]

CCK8 assay to determine the impact of Dehydrocorydaline (DHC) (5, 10, 30, 50, 80, 100, 150 μM) on HPFs viability.
Eur J Pharmacol

Eriodictyol inhibits glioblastoma migration and invasion by reversing EMT via downregulation of the P38 MAPK/GSK-3β/ZEB1 pathway. [Abstract]

2021 Jun 5:900:174069. PMID: 33811837
Int Immunopharmacol

MCPIP1 modulates host antifungal immunity and exacerbates lethal Candida albicans infection. [Abstract]

2026 Jul 15:181:116715. PMID: 42066549
Int Immunopharmacol

Salvianolic acid a inhibits neuroinflammation and ameliorates Alzheimer's disease pathology via the p38 MAPK/NF-κB pathway based on network pharmacology and experimental validation. [Abstract]

2026 Jun 1:178:116594. PMID: 41930874
Int Immunopharmacol

Eupatilin suppresses osteoclastogenesis and periodontal bone loss by inhibiting the MAPKs/Siglec-15 pathway. [Abstract]

2024 Sep 30:139:112720. PMID: 39047450
Bone Joint Res

Melatonin induces RAW264.7 cell apoptosis via the BMAL1/ROS/MAPK-p38 pathway to improve postmenopausal osteoporosis. [Abstract]

2023 Nov 1;12(11):677-690. PMID: 37907083
Arthritis Res Ther

Roles of VWA1 and Rac2 in compensatory and decompensatory responses via FGF9/FGFR3 and p38 MAPK signaling pathways in condylar chondrocytes under distinct mechanical stress. [Abstract]

2025 May 31;27(1):118. PMID: 40450276
Front Pharmacol

Inhibition of ASIC1a-Mediated ERS Improves the Activation of HSCs and Copper Transport Under Copper Load. [Abstract]

2021 May 31:12:653272. PMID: 34135753
J Inflamm Res

The Effects of Adoptively Transferred IL-23/IL-18-Polarized Neutrophils on Tumor and Collagen-Induced Arthritis in Mice. [Abstract]

2021 Sep 16;14:4669-4686. PMID: 34557012
FASEB J

USP44 Regulates Chemoresistance Induced by ROS and the MAPK/NF-κB Pathway Through the Stabilization of ITGB4 in Gastric Cancer. [Abstract]

2025 Aug 31;39(16):e70945. PMID: 40824171
J Nutr

A-Type Cinnamon Procyanidin Oligomers Protect Against 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Neurotoxicity in Mice Through Inhibiting the P38 Mitogen-Activated Protein Kinase/P53/BCL-2 Associated X Protein Signaling Pathway. [Abstract]

2020 Jul 1;150(7):1731-1737. PMID: 32386222
Front Biosci (Landmark Ed)

NELFCD Promotes Colon Cancer Progression by Regulating the DUSP2-p38 Axis. [Abstract]

2025 Apr 25;30(4):25221. PMID: 40302325
J Food Sci

Curcumin Alleviates DON-Induced Intestinal Epithelial Barrier Disruption by Improving Ribotoxic Stress-Associated p38 Pathway-Mediated TJ Injury, Apoptosis, and Cell Cycle Arrest. [Abstract]

2025 Apr;90(4):e70217. PMID: 40271829
J Cell Physiol

STRIP2 silencing inhibits vascular smooth muscle cell proliferation and migration via P38-AKT-MMP-2 signaling pathway. [Abstract]

2019 Dec;234(12):22463-22476. PMID: 31093976
Genomics

MiR-100-5p as a biomarker in hand-arm vibration disease: Mediating pathogenesis through angiogenesis suppression. [Abstract]

2025 Jul;117(4):111067. PMID: 40460899
Mol Carcinog

ITGB3-enriched extracellular vesicles mediate the formation of osteoclastic pre-metastatic niche to promote lung adenocarcinoma bone metastasis. [Abstract]

2024 Nov;63(11):2190-2204. PMID: 39136603
Andrology

Expression of IL-33 in rodent testes and its role in Leydig cell steroidogenesis and aging. [Abstract]

2025 Jun 16. PMID: 40521800
Tissue Cell

Placental mesenchymal stem cells-secreted proenkephalin suppresses the p38 MAPK signaling to block hyperproliferation of keloid fibroblasts. [Abstract]

2023 Dec:85:102218. PMID: 37913601
Exp Eye Res

Embryonic stem cell extracellular vesicles reverse the senescence of retinal pigment epithelial cells by the p38MAPK pathway. [Abstract]

2023 Feb:227:109365. PMID: 36577484
BMC Musculoskelet Disord

Knockdown of PAR2 alleviates cancer-induced bone pain by inhibiting the activation of astrocytes and the ERK pathway. [Abstract]

2022 May 30;23(1):514. PMID: 35637468
Infect Genet Evol

Knockdown of GBP1 inhibits BCG-induced apoptosis in macrophage RAW 264.7 cells via p38/JNK pathway. [Abstract]

2022 Jan:97:105158. PMID: 34826624
J Mol Histol

Nogo-A-Δ20/EphA4 interaction antagonizes apoptosis of neural stem cells by integrating p38 and JNK MAPK signaling. [Abstract]

2021 Jun;52(3):521-537. PMID: 33555537
Biochem Biophys Res Commun

Deficiency of unc-51 like kinase 1 (Ulk1) protects against mice traumatic brain injury (TBI) by suppression of p38 and JNK pathway. [Abstract]

2018 Sep 5;503(2):467-473. PMID: 29680658

Dehydrocorydaline nitrate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 5;503(2):467-473. [Abstract]

The increase of Bcl-2 and decrease of Bax, cleaved Caspase-3 and PARP induced by Ulk1- inhibition in LPS-exposed AST are abrogated by the Dehydrocorydaline chloride (DHC) or Anisomycin (ANS) pre-treatment
Biochem Biophys Res Commun

NFATc3 deficiency protects against high fat diet (HFD)-induced hypothalamus inflammation and apoptosis via p38 and JNK suppression. [Abstract]

2018 May 23;499(4):743-750. PMID: 29596828

Dehydrocorydaline nitrate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2018 May 23;499(4):743-750. [Abstract]

Astrocytes are cultured with p38 and JNK activator of DHC and ANS, respectively, at the indicated concentrations for 24 h. Then, all cells are harvested for western blot analysis of p-p38 and p-JNK. Astrocytes are pretreated with DHC (500 nM) or ANS (10 mM) for 24 h, followed by Fru (5 mM) for an additional 24 h.
Eur J Histochem

Adipose-derived stem cells promote the recovery of intestinal barrier function by inhibiting the p38 MAPK signaling pathway. [Abstract]

2025 Jan 21;69(1). PMID: 39836101
J Clin Neurosci

Effects of p38 MAPK signaling pathway on cognitive function and recovery of neuronal function after hypoxic-ischemic brain injury in newborn rats. [Abstract]

2020 Aug:78:365-370. PMID: 32360159
J Cardiothorac Surg

Aconitine promotes ROS-activated P38/MAPK/Nrf2 pathway to inhibit autophagy and promote myocardial injury. [Abstract]

2024 Dec 20;19(1):665. PMID: 39707526
bioRxiv

2026 May 7.
Bioengineered

Deleted in lymphocytic leukemia 2 induces retinoic acid receptor beta promoter methylation and mitogen activated kinase-like protein activation to enhance viability and mobility of colorectal cancer cells. [Abstract]

2022 May;13(5):12847-12862. PMID: 35611845
Aging (Albany NY)

Short-term high-fat diet favors the appearances of apoptosis and gliosis by activation of ERK1/2/p38MAPK pathways in brain. [Abstract]

2021 Oct 7;13(19):23133-23148. PMID: 34620734
Comput Math Methods Med

Efficacy of Low Molecular Heparin on Preeclampsia by Inhibiting Apoptosis of Trophoblasts via the p38MAPK Signaling Pathway. [Abstract]

2021 Aug 2:2021:3337514. PMID: 34394705
Aging (Albany NY)

CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis. [Abstract]

2021 May 11;13(9):12780-12799. PMID: 33973871
Research Square Preprint

2020 Dec.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (116.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3340 mL

11.6702 mL

23.3405 mL

5 mM

0.4668 mL

2.3340 mL

4.6681 mL

10 mM

0.2334 mL

1.1670 mL

2.3340 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (348 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Nonaka M, et al. Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts. Malaria journal. 2018 Jun 25;17(1):244. [Content Brief]

[2]. Tayama Y, et al. Anti-Trypanosoma cruzi activity of Coptis rhizome extract and its constituents. Tropical medicine and health. 2023 Mar 01;51(1):12.

[3]. Yoo M, et al. Dehydrocorydaline promotes myogenic differentiation via p38 MAPK activation. Molecular medicine reports. 2016 Oct;14(4):3029-36. [Content Brief]

[4]. Xu Z, et al. Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells. The American journal of Chinese medicine. 2012;40(1):177-85. [Content Brief]

[5]. Jin L, et al. Dehydrocorydaline induced antidepressant-like effect in a chronic unpredictable mild stress mouse model via inhibiting uptake-2 monoamine transporters. European journal of pharmacology. 2019 Dec 01;864:172725.

[6]. Yin ZY, et al. Antinociceptive effects of dehydrocorydaline in mouse models of inflammatory pain involve the opioid receptor and inflammatory cytokines. Scientific reports. 2016 Jun 07;6:27129. [Content Brief]

[7]. Huo W, et al. Dehydrocorydaline attenuates bone cancer pain by shifting microglial M1/M2 polarization toward the M2 phenotype. Mol Pain. 2018 Jan-Dec;14:1744806918781733.

[8]. Kim G, et al. Antibacterial Activity and Multi-Targeting Mechanism of Dehydrocorydaline From Corydalis turtschaninovii Bess. Against Listeria monocytogenes. Front Microbiol. 2022 Jan 11;12:799094.

[9]. Wen B, et al. Antiatherosclerotic effect of dehydrocorydaline on ApoE mice: inhibition of macrophage inflammation. Acta pharmacologica Sinica. 2022 Jun;43(6):1408-1418. [Content Brief]

[10]. Li Y, et al. Dehydrocorydaline Protects Against Sepsis-Induced Myocardial Injury Through Modulating the TRAF6/NF-κB Pathway. Front Pharmacol. 2021 Aug 19;12:709604. [Content Brief]

[11]. Dang YY, et al. Dehydrocorydaline maintains the vascular smooth muscle cell contractile phenotype by upregulating Spta1. Acta pharmacologica Sinica. 2025 May;46(5):1303-1316.

[12]. He J, et al. Dehydrocorydaline attenuates bleomycin-induced pulmonary fibrosis by inhibiting fibroblast activation. Respir Res. 2025 Apr 12;26(1):136. [Content Brief]

[13]. Shen J, et al. Multi-targeted inhibition of NF-κB signaling underlies the anti-osteoarthritic effects of BGJXF and its key component dehydrocorydaline. Front Med (Lausanne). 2026 Jan 9;12:1676071.

[14]. Hu H, et al. Dehydrocorydaline inhibits cell proliferation, migration and invasion via suppressing MEK1/2-ERK1/2 cascade in melanoma. Onco Targets Ther. 2019 Jul 2;12:5163-5175. [Content Brief]

[15]. Lee J, et al. Anti-Metastatic Effect of Dehydrocorydaline on H1299 Non-Small Cell Lung Carcinoma Cells via Inhibition of Matrix Metalloproteinases and B Cell Lymphoma 2. Phytotherapy research : PTR. 2017 Mar;31(3):441-448.

[16]. Hou BL, et al. Analgesic Effect of Dehydrocorydaline on Chronic Constriction Injury-Induced Neuropathic Pain via Alleviating Neuroinflammation. Chinese journal of integrative medicine. 2025 Jun;31(6):499-505.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3340 mL	11.6702 mL	23.3405 mL	58.3512 mL
	5 mM	0.4668 mL	2.3340 mL	4.6681 mL	11.6702 mL
	10 mM	0.2334 mL	1.1670 mL	2.3340 mL	5.8351 mL
	15 mM	0.1556 mL	0.7780 mL	1.5560 mL	3.8901 mL
	20 mM	0.1167 mL	0.5835 mL	1.1670 mL	2.9176 mL
	25 mM	0.0934 mL	0.4668 mL	0.9336 mL	2.3340 mL
	30 mM	0.0778 mL	0.3890 mL	0.7780 mL	1.9450 mL
	40 mM	0.0584 mL	0.2918 mL	0.5835 mL	1.4588 mL
	50 mM	0.0467 mL	0.2334 mL	0.4668 mL	1.1670 mL
	60 mM	0.0389 mL	0.1945 mL	0.3890 mL	0.9725 mL
	80 mM	0.0292 mL	0.1459 mL	0.2918 mL	0.7294 mL
	100 mM	0.0233 mL	0.1167 mL	0.2334 mL	0.5835 mL

Bioz

Dehydrocorydaline nitrate

45 Cited Publications

100 mg Get Quote

Subscribe to the

MedChemExpress

Newsletter

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Dehydrocorydaline nitrate

Publications Citing Use of MedChemExpress (MCE) Dehydrocorydaline nitrate

All Cholinesterase (ChE) Isoforms

All Parasite Isoforms

All Bcl-2 Family Isoforms

All Caspase Isoforms

All PARP Isoforms

All p38 MAPK Isoforms

All NOD-like Receptor (NLR) Isoforms

All 5-HT Receptor Isoforms

All Dopamine Receptor Isoforms

Biological Activity

Description

IC₅₀ & Target

Cellular Effect

In Vitro

In Vivo

Chemical Information

Publications (45)

Solvent & Solubility

In Vitro:

In Vivo:

In Vivo Dissolution Calculator

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

Keywords

Your Recently Viewed Products

Dehydrocorydaline nitrate

Publications Citing Use of MedChemExpress (MCE) Dehydrocorydaline nitrate

All Cholinesterase (ChE) Isoforms

All Parasite Isoforms

All Bcl-2 Family Isoforms

All Caspase Isoforms

All PARP Isoforms

All p38 MAPK Isoforms

All NOD-like Receptor (NLR) Isoforms

All 5-HT Receptor Isoforms

All Dopamine Receptor Isoforms

Biological Activity

Description

IC50 & Target

Cellular Effect

In Vitro

In Vivo

Chemical Information

Publications (45)

Solvent & Solubility

In Vitro:

In Vivo:

In Vivo Dissolution Calculator

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table