Nlrp3 - NLR family, pyrin domain containing 3 Gene

Also Known as FCU; MWS; FCAS; Cias1; Mmig1; NALP3; Pypaf1; AII/AVP; AGTAVPRL

Species: Mus musculus

Gene Type: protein coding

Gene ID: 216799

About Nlrp3

Chromosome 11: 59,432,394-59,457,782 forward strand.GRCm39:CM001004.3

This gene has 3 transcripts (splice variants), 87 orthologues, 22 paralogues and is associated with 95 phenotypes. Broad expression in spleen adult (RPKM 1.1), lung adult (RPKM .8) and 25 other tissues.

Summary

Enables DNA-binding transcription factor binding activity and sequence-specific DNA binding activity. Involved in several processes, including positive regulation of T-helper cell differentiation; positive regulation of cytokine production; and response to bacterium. Acts upstream of or within several processes, including NLRP3 inflammasome complex assembly; activation of cysteine-type endopeptidase activity involved in apoptotic process; and defense response to virus. Located in cytoplasm and nucleus. Part of NLRP3 inflammasome complex. Is expressed in central nervous system and retina. Used to study CINCA Syndrome; familial cold autoinflammatory syndrome 1; and non-alcoholic fatty liver disease. Human ortholog(s) of this gene implicated in CINCA Syndrome; Muckle-Wells syndrome; autosomal dominant nonsyndromic deafness 34; familial cold autoinflammatory syndrome 1; and urticaria. Orthologous to human NLRP3 (NLR family pyrin domain containing 3). [provided by Alliance of Genome Resources, Apr 2022]

Nlrp3 Products (2)

mRNA	Protein	Name
NM_001359638.1	NP_001346567.1	NACHT, LRR and PYD domains-containing protein 3
NM_145827.4	NP_665826.1	NACHT, LRR and PYD domains-containing protein 3

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables ATP hydrolysis activity	IDA IDA: Inferred from direct assay	34861190	MGI
enables DNA-binding transcription factor binding	IDA IDA: Inferred from direct assay	26098997	MGI
enables molecular sensor activity	IDA IDA: Inferred from direct assay	18403674	MGI
enables phosphatidylinositol phosphate binding	IDA IDA: Inferred from direct assay	34861190	MGI
enables phosphatidylinositol-4-phosphate binding	IDA IDA: Inferred from direct assay	30487600	MGI
enables protein binding	IPI IPI: Inferred from physical interaction	22461501	MGI
enables sequence-specific DNA binding	IDA IDA: Inferred from direct assay	26098997	MGI
enables signaling adaptor activity	IDA IDA: Inferred from direct assay	26173909	MGI

Biological Process GO Annotation	Evidence	References	Source
involved in NLRP3 inflammasome complex assembly	IDA IDA: Inferred from direct assay	23582325	MGI
acts upstream of or within NLRP3 inflammasome complex assembly	IMP IMP: Inferred from mutant phenotype	22753929	MGI
acts upstream of or within activation of cysteine-type endopeptidase activity involved in apoptotic process	IMP IMP: Inferred from mutant phenotype	17008311	MGI
acts upstream of or within acute inflammatory response	IDA IDA: Inferred from direct assay	30248149	MGI
involved in cellular response to lipopolysaccharide	IDA IDA: Inferred from direct assay	28847925	MGI
involved in cellular response to peptidoglycan	IMP IMP: Inferred from mutant phenotype	27374331	MGI
involved in defense response to Gram-positive bacterium	IMP IMP: Inferred from mutant phenotype	27374331	MGI
acts upstream of or within defense response to virus	IMP IMP: Inferred from mutant phenotype	17008311	MGI
involved in detection of biotic stimulus	IDA IDA: Inferred from direct assay	18403674	MGI
involved in inflammatory response	IDA IDA: Inferred from direct assay	28847925	MGI
acts upstream of or within interleukin-1 beta production	IDA IDA: Inferred from direct assay	30248149	MGI
acts upstream of interleukin-1 beta production	IGI IGI: Inferred from genetic interaction	22002608	MGI
acts upstream of or within interleukin-1 beta production	IMP IMP: Inferred from mutant phenotype	22753929	MGI
acts upstream of or within interleukin-1 production	IMP IMP: Inferred from mutant phenotype	17008311	MGI
acts upstream of or within interleukin-18 production	IMP IMP: Inferred from mutant phenotype	17008311	MGI
acts upstream of or within macrophage cytokine production	IDA IDA: Inferred from direct assay	30248149	MGI
acts upstream of or within macrophage cytokine production	IMP IMP: Inferred from mutant phenotype	22753929	MGI
involved in positive regulation of T-helper 17 cell differentiation	IMP IMP: Inferred from mutant phenotype	26098997	MGI
involved in positive regulation of T-helper 2 cell cytokine production	IMP IMP: Inferred from mutant phenotype	26098997	MGI
involved in positive regulation of T-helper 2 cell differentiation	IMP IMP: Inferred from mutant phenotype	26098997	MGI
involved in positive regulation of cytokine production involved in immune response	IMP IMP: Inferred from mutant phenotype	27374331	MGI
involved in positive regulation of inflammatory response	IDA IDA: Inferred from direct assay	34861190	MGI
involved in positive regulation of interleukin-1 beta production	IDA IDA: Inferred from direct assay	23582325	MGI
acts upstream of or within positive regulation of interleukin-1 beta production	IGI IGI: Inferred from genetic interaction	22002608	MGI
involved in positive regulation of interleukin-1 beta production	IMP IMP: Inferred from mutant phenotype	27374331	MGI
involved in positive regulation of interleukin-13 production	IMP IMP: Inferred from mutant phenotype	26098997	MGI
involved in positive regulation of interleukin-4 production	IDA IDA: Inferred from direct assay	26098997	MGI
involved in positive regulation of interleukin-5 production	IMP IMP: Inferred from mutant phenotype	26098997	MGI
involved in positive regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	26098997	MGI
involved in positive regulation of type 2 immune response	IMP IMP: Inferred from mutant phenotype	26098997	MGI
involved in protein homooligomerization	IDA IDA: Inferred from direct assay	34861190	MGI
acts upstream of or within regulation of inflammatory response	IMP IMP: Inferred from mutant phenotype	22297845	MGI

Cellular Component GO Annotation	Evidence	References	Source
part of NLRP3 inflammasome complex	IMP IMP: Inferred from mutant phenotype	27374331	MGI
part of canonical inflammasome complex	IDA IDA: Inferred from direct assay	30248149	MGI
located in cytoplasm	IDA IDA: Inferred from direct assay	26098997	MGI
located in interphase microtubule organizing center	IDA IDA: Inferred from direct assay	34861190	MGI
is active in membrane	IDA IDA: Inferred from direct assay	26173909	MGI
located in membrane	IDA IDA: Inferred from direct assay	34861190	MGI
located in microtubule organizing center	IDA IDA: Inferred from direct assay	28656979	MGI
located in mitochondrion	IDA IDA: Inferred from direct assay	23582325	MGI
located in nucleus	IDA IDA: Inferred from direct assay	26098997	MGI

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

Nlrp3 Protein Structure

Pyrin_NALPs

FISNA

NACHT

NOD2_WH

LRR_RI

0
200
400
600
800
1033 a.a.

Protein Preferred Names	Protein Names
NACHT, LRR and PYD domains-containing protein 3	NACHT/LRR/pyrin domain-containing protein 3 PYRIN-containing APAF1-like protein 1 cold autoinflammatory syndrome 1 protein homolog cryopyrin mast cell maturation-associated-inducible protein 1

Nlrp3 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	Nlrp3	Q8R4B8	Casp1	Mus musculus	P29452	Anti Bait CoIP	27993998
Intra	Nlrp3	Q8R4B8	Pycard	Mus musculus	Q9EPB4	Anti Bait CoIP	27993998

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant Nlrp3 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P71598	NLRP3 Protein, Mouse (His-SUMO)	Q8R4B8-1 (M1-R153)	≥ 90%, as determined by reducing SDS-PAGE.

Related Agents

Approval
Clinical Phase
Pre-clinical Phase

Approval

Bioactive Molecules (13)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-B2176	ATP	Activator	99.88%	Unkown
HY-132591A	Inclisiran sodium	Inhibitor	99.08%	Unkown
HY-132591	Inclisiran	Inhibitor	99.48%	Unkown
HY-B0116	Stavudine	Inhibitor	99.89%	Yes
HY-14806B	Teneligliptin hydrobromide hydrate	Inhibitor	99.97%	Unkown
HY-16059	Arglabin	Inhibitor	99.87%	Unkown
HY-B1239	Drofenine hydrochloride		98.10%	Unkown
HY-17355B	Dexpramipexole	Inhibitor	99.56%	Yes
HY-B0516	Articaine hydrochloride	Inhibitor	99.38%	Unkown
HY-B0516A	Articaine	Inhibitor	/	Unkown
HY-B0516R	Articaine hydrochloride (Standard)	Inhibitor	/	Unkown
HY-B1239A	Drofenine		/	Unkown
HY-B0116A	Stavudine sodium	Inhibitor	99.40%	Yes

Clinical Phase

Bioactive Molecules (6)

Cat. No.	Product Name	Effect	Purity	Phase
HY-17629	Dapansutrile	Inhibitor	99.92%	Phase 3
HY-156034	JT001	Inhibitor	/	Phase 3
HY-168264	MC1	Inhibitor	/	Phase 3
HY-13535	ATN-161	Inhibitor	99.36%	Phase 2
HY-139396	BMS-986299	Agonist	99.96%	Phase 1
HY-19309	ABT-546 hydrochloride	Inhibitor	/	Phase 1

Inhibitory Antibodies (1)

Cat. No.	Product Name	Effect	Purity	Phase
HY-P99555	Tomaralimab	Inhibitor	99.42%	Phase 2

Pre-clinical Phase

Bioactive Molecules (178)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-12815	MCC950	Inhibitor	99.78%	Unkown
HY-127019	Nigericin	Activator	99.64%	Unkown
HY-100381	Nigericin sodium salt	Activator	99.60%	Unkown
HY-12815A	MCC950 sodium	Inhibitor	99.75%	Unkown
HY-100573	Necrosulfonamide	Inhibitor	98.61%	Unkown
HY-12497	ANA-12	Inhibitor	99.90%	Unkown
HY-116084	Trimethylamine N-oxide	Activator	98.0%	Unkown
HY-144226	NLRP3/AIM2-IN-3	Inhibitor	98.23%	Unkown
HY-103666	CY-09	Inhibitor	99.01%	Unkown
HY-101092	QS-21-Api	Activator	99.73%	Unkown
HY-156798	ADS032	Inhibitor	99.89%	Unkown
HY-113298	Citraconic acid	Inhibitor	99.08%	Unkown
HY-N0373	Licochalcone B	Inhibitor	99.91%	Unkown
HY-N3005	Britannin	Inhibitor	99.93%	Unkown
HY-108915	Trimethylamine N-oxide dihydrate	Activator	99.86%	Unkown
HY-N0633	Muscone	Inhibitor	98.0%	Unkown
HY-N0806	Sweroside	Inhibitor	99.76%	Unkown
HY-155876	NVP-DFV890	Antagonist	99.97%	Unkown
HY-100581	CORM-3	Inhibitor	97.5%	Unkown
HY-N0496	Ruscogenin	Inhibitor	99.85%	Unkown
HY-132831	Selnoflast	Inhibitor	99.46%	Unkown
HY-W019670	N4-Acetylcytidine	Activator	99.65%	Unkown
HY-N2414	Periplogenin	Inhibitor	≥99.70%	Unkown
HY-161834	RG100204	Inhibitor	99.50%	Unkown
HY-N5042	Forsythoside I	Inhibitor	99.94%	Unkown
HY-159520	Ofirnoflastum	Inhibitor	99.93%	Unkown
HY-N5063	Plantainoside D	Inhibitor	99.15%	Unkown
HY-169996	NP3-253	Inhibitor	99.78%	Unkown
HY-173154B	NK7-902 diTFA	Inhibitor	99.88%	Unkown
HY-162333	BAL-0028	Inhibitor	99.86%	Unkown
HY-130494	(±)11(12)-EET	Inhibitor	98.30%	Unkown
HY-N0257	Epimedin A	Inhibitor	99.43%	Unkown
HY-P10227	Xelafaslatide	Inhibitor	99.88%	Unkown
HY-152670	JC2-11	Inhibitor	98.03%	Unkown
HY-119684	Maresin 2	Inhibitor	97.20%	Unkown
HY-78263	MNS		99.55%	Unkown
HY-W082785A	L6H21	Inhibitor	99.19%	Unkown
HY-N0381	Maackiain	Activator	99.83%	Unkown
HY-N1939	Icariside I	Agonist	98.76%	Unkown
HY-B2176I	ATP disodium salt, 100 mM Solution, PCR Grade	Activator	/	Unkown
HY-N1431	Tabersonine	Inhibitor	98.13%	Unkown
HY-137888A	Oxidized ATP trisodium salt	Inhibitor	98.0%	Unkown
HY-117878	ML345	Inhibitor	99.37%	Unkown
HY-164304	INF 195	Inhibitor	99.58%	Unkown
HY-144223	NLRP3-IN-94	Inhibitor	99.93%	Unkown
HY-150206	NLRP3-IN-13	Inhibitor	98.03%	Unkown
HY-P2310	Defensin HNP-1 human	Activator	98.12%	Unkown
HY-N6893	Ergolide	Inhibitor	99.48%	Unkown
HY-N3242	Moronic acid	Inhibitor	98.6%	Unkown
HY-101868	INF39	Inhibitor	99.84%	Unkown
HY-153261	NLRP3-IN-17	Inhibitor	99.02%	Unkown
HY-N0699	Daphnoretin	Inhibitor	99.83%	Unkown
HY-120007	JC124	Inhibitor	99.72%	Unkown
HY-W158030	2-Guanidinobezimidazole	Agonist	95.74%	Unkown
HY-163358	SLC3037	Inhibitor	99.85%	Unkown
HY-176860	NEK7 degrader-1	Activator	99.94%	Unkown
HY-N2485	4'-Methoxyresveratrol	Activator	99.48%	Unkown
HY-122920	Soyasaponin II	Inhibitor	99.81%	Unkown
HY-163159	NP3-562	Inhibitor	98.16%	Unkown
HY-130252	YQ128	Inhibitor	99.74%	Unkown
HY-N2128	Nigakinone	Inhibitor	99.95%	Unkown
HY-137245	Emlenoflast	Inhibitor	99.30%	Unkown
HY-N2157	Pteryxin	Inhibitor	99.55%	Unkown
HY-155751	HMGB1-IN-1	Inhibitor	98.40%	Unkown
HY-N7695	Physalin B	Inhibitor	97.20%	Unkown
HY-156034A	JT001 sodium	Inhibitor	98.39%	Unkown
HY-N8599	Cichoriin	Inhibitor	99.9%	Unkown
HY-160757	NLRP3 modulator 7	Modulator	/	Unkown
HY-146765	AMS-17	Inhibitor	98.09%	Unkown
HY-179494	M464	Inhibitor	99.58%	Unkown
HY-161727	P2Y14R antagonist 1	Inhibitor	98.76%	Unkown
HY-126941	Hecogenin acetate	Inhibitor	98.57%	Unkown
HY-172941	VEN-02XX	Inhibitor	99.66%	Unkown
HY-161627	Anemoside A3-methyl 6-aminohexanoate	Inhibitor	98.62%	Unkown
HY-175278	BAL-1516	Inhibitor	/	Unkown
HY-174270	NLRP3-IN-79	Inhibitor	99.10%	Unkown
HY-W011082	NLRP3-IN-2	Inhibitor	98.00%	Unkown
HY-164853	Kanglexin	Inhibitor	99.65%	Unkown
HY-137245A	Emlenoflast sodium	Inhibitor	98.31%	Unkown
HY-117432	JC-171	Inhibitor	98.06%	Unkown
HY-131040	NLRP3-IN-NBC6	Inhibitor	99.40%	Unkown
HY-161297	NT-0249	Inhibitor	/	Unkown
HY-145087A	NP3-146 sodium	Inhibitor	99.26%	Unkown
HY-P6084	RP-220	Inhibitor	/	Unkown
HY-W017087	1,2,4-Trimethoxybenzene	Inhibitor	/	Unkown
HY-121988	INF4E	Inhibitor	99.38%	Unkown
HY-179421	PROTAC HDAC6 degrader 7	Inhibitor	/	Unkown
HY-186072	NT-0527	Inhibitor	99.80%	Unkown
HY-148187	NLRP3-IN-11	Inhibitor	98.79%	Unkown
HY-153554	NLRP3-IN-18	Inhibitor	98.06%	Unkown
HY-162588	MC-ND-18	Degrader	/	Unkown
HY-N10173	(+)-Epiloliolide	Inhibitor	95.0%	Unkown
HY-W010253	Benzylurea	Modulator	99.94%	Unkown
HY-N10359	Isoandrographolide	Inhibitor	98.34%	Unkown
HY-150965	NDT-30805	Inhibitor	/	Unkown
HY-178898	7-Oxogedunin	Inhibitor	/	Unkown
HY-149087	MR2938	Inhibitor	/	Unkown
HY-159482	SBN-284	Inhibitor	/	Unkown
HY-175814	NLRP3-IN-83	Inhibitor	/	Unkown
HY-169227	NLRP3-IN-55	Inhibitor	/	Unkown
HY-179132	TN-101	Inhibitor	/	Unkown
HY-179682	HDAC3 degrader-1	Inhibitor	/	Unkown
HY-145087	NP3-146	Inhibitor	/	Unkown
HY-145910	NLRP3-IN-6	Inhibitor	/	Unkown
HY-175645	NLRP3/URAT1-IN-1	Inhibitor	/	Unkown
HY-163744	NLRP3-IN-42	Inhibitor	/	Unkown
HY-183296	NLRP3-IN-91	Inhibitor	/	Unkown
HY-135283	ABT-546	Inhibitor	/	Unkown
HY-185311	NEK7 degrader-3	Inhibitor	/	Unkown
HY-172102	NLRP3-IN-68	Inhibitor	/	Unkown
HY-169188	NLRP3-IN-50	Inhibitor	/	Unkown
HY-178953	NLRP3-IN-84	Inhibitor	/	Unkown
HY-103715	NLRP3 modulator 1	Modulator	/	Unkown
HY-N10312	Yadanzigan	Inhibitor	/	Unkown
HY-149122	NLRP3-IN-15	Inhibitor	/	Unkown
HY-N20708	Chiisanogenin	Inhibitor	/	Unkown
HY-163695	NLRP3-IN-41	Inhibitor	/	Unkown
HY-180168	NQO2 activator-1	Inhibitor	/	Unkown
HY-181809	BLT1-IN-1	Inhibitor	/	Unkown
HY-175464	NLRP3-IN-82	Inhibitor	/	Unkown
HY-N17383	Ligusticum cycloprolactam	Inhibitor	/	Unkown
HY-173294	NLRP3-IN-74	Inhibitor	/	Unkown
HY-132892	NLRP3-IN-4	Inhibitor	/	Unkown
HY-172134	NLRP3-IN-70	Inhibitor	/	Unkown
HY-159007	BD-AcAc2	Inhibitor	/	Unkown
HY-182102	NLRP3-IN-88	Inhibitor	/	Unkown
HY-149123	NLRP3-IN-16	Inhibitor	/	Unkown
HY-179131	TN-783	Inhibitor	/	Unkown
HY-183283	CAII-IN-15	Inhibitor	/	Unkown
HY-180916	CAII-IN-11	Inhibitor	/	Unkown
HY-182779	JNUTS013	Inhibitor	/	Unkown
HY-168594	α-Glucosidase/NLRP3-IN-1	Inhibitor	/	Unkown
HY-184357	NLRP3-IN-92	Inhibitor	/	Unkown
HY-145911	NLRP3-IN-7	Inhibitor	/	Unkown
HY-183995	SH-26	Inhibitor	/	Unkown
HY-179387	CXCR2-IN-3	Inhibitor	/	Unkown
HY-161329	NLRP3-IN-32	Inhibitor	/	Unkown
HY-113298R	Citraconic acid (Standard)	Inhibitor	/	Unkown
HY-159898	AChE/BChE-IN-23	Inhibitor	/	Unkown
HY-132831C	Selnoflast monopotassium	Inhibitor	/	Unkown
HY-146802	NLRP3-IN-9	Inhibitor	/	Unkown
HY-177559	NEK7 degrader-2	Inhibitor	/	Unkown
HY-168737	NEK7-IN-2	Inhibitor	/	Unkown
HY-N19847	LYRM03	Inhibitor	/	Unkown
HY-184711	Z1456467176	Inhibitor	/	Unkown
HY-182778	Anti-inflammatory agent 114	Inhibitor	/	Unkown
HY-401677	Abdenoflast	Modulator	/	Unkown
HY-168009	NLRP3-IN-45	Inhibitor	/	Unkown
HY-171589	NLRP3-IN-77	Inhibitor	/	Unkown
HY-179429	NLRP3-IN-85	Inhibitor	/	Unkown
HY-177128	Sebrinoflast	Inhibitor	/	Unkown
HY-183796	GFAP/NF-κB/APOE/NLRP3-IN-1	Inhibitor	/	Unkown
HY-146594	NLRP3-IN-8	Inhibitor	/	Unkown
HY-149121	NLRP3-IN-14	Inhibitor	/	Unkown
HY-180830	NLRP3-IN-86	Inhibitor	/	Unkown
HY-163494	NLRP3-IN-36	Inhibitor	/	Unkown
HY-N10650	Caulerpin	Inhibitor	/	Unkown
HY-181488	NLRP3-IN-87	Inhibitor	/	Unkown
HY-N6189	MBL-1	Inhibitor	/	Unkown
HY-182803	NLRP3-IN-90	Inhibitor	/	Unkown
HY-163497	NLRP3-IN-38	Inhibitor	/	Unkown
HY-174312	NLRP3-IN-81	Inhibitor	/	Unkown
HY-184651	NLRP3-IN-93	Inhibitor	/	Unkown
HY-180890	PPARγ agonist-21	Inhibitor	/	Unkown
HY-178950	Hck-IN-3	Inhibitor	/	Unkown
HY-182287	PRDX1-IN-4	Inhibitor	/	Unkown
HY-P11581	MNP2	Inhibitor	/	Unkown
HY-182794	NLRP3-IN-89	Inhibitor	/	Unkown
HY-172778	HNW005	Inhibitor	/	Unkown
HY-179024	NP3-742	Inhibitor	/	Unkown
HY-182380	ODZ10117	Inhibitor	/	Unkown
HY-165316	Myristoyllysophosphatidylcholine	Inhibitor	/	Unkown
HY-184844	NLRP3 modulator 8-1	Inhibitor	/	Unkown
HY-183889	p-Ethoxychrysoidin	Inhibitor	/	Unkown
HY-119684S	Maresin 2	Inhibitor	/	Unkown
HY-W929472	13(S)-HOTrE	Inhibitor	99.9%	Unkown
HY-N1431A	Tabersonine hydrochloride	Inhibitor	98.55%	Unkown
HY-184882	NLRP3-IN-95	Inhibitor	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Homo sapiens	Nlrp3	NCBI	NCBI:114548